Magnetite Nanoparticles Functionalized with RNases against Intracellular Infection of <i>Pseudomonas aeruginosa</i>

• Main Authors: Nathaly Rangel-Muñoz (Author), Alejandra Suarez-Arnedo (Author), Raúl Anguita (Author), Guillem Prats-Ejarque (Author), Johann F. Osma (Author), Carolina Muñoz-Camargo (Author), Ester Boix (Author), Juan C. Cruz (Author), Vivian A. Salazar (Author)
• Format: Book
• Published: MDPI AG, 2020-07-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.3390/pharmaceutics12070631; 1999-4923
• Keywords: <i>Pseudomonas aeruginosa</i>; ribonucleases; magnetite nanoparticles; antimicrobials; Pharmacy and materia medica; RS1-441
• Source: Pharmaceutics, Vol 12, Iss 7, p 631 (2020)

Current treatments against bacterial infections have severe limitations, mainly due to the emergence of resistance to conventional antibiotics. In the specific case of <i>Pseudomonas</i> <i>aeruginosa</i> strains, they have shown a number of resistance mechanisms to counter most antibiotics. Human secretory RNases from the RNase A superfamily are proteins involved in a wide variety of biological functions, including antimicrobial activity. The objective of this work was to explore the intracellular antimicrobial action of an RNase 3/1 hybrid protein that combines RNase 1 high catalytic and RNase 3 bactericidal activities. To achieve this, we immobilized the RNase 3/1 hybrid on Polyetheramine (PEA)-modified magnetite nanoparticles (MNPs). The obtained nanobioconjugates were tested in macrophage-derived THP-1 cells infected with <i>Pseudomonas aeruginosa</i> PAO1. The obtained results show high antimicrobial activity of the functionalized hybrid protein (MNP-RNase 3/1) against the intracellular growth of <i>P. aeruginosa</i> of the functionalized hybrid protein. Moreover, the immobilization of RNase 3/1 enhances its antimicrobial and cell-penetrating activities without generating any significant cell damage. Considering the observed antibacterial activity, the immobilization of the RNase A superfamily and derived proteins represents an innovative approach for the development of new strategies using nanoparticles to deliver antimicrobials that counteract <i>P. aeruginosa</i> intracellular infection.