Changes in the content of interleukins in the serum of male and female rates with different resistance to acute hypoxic hypoxia under immobilization stress

• Main Authors: O. V. Denefil (Author), Iu. M. Ordynskyi (Author)
• Format: Book
• Published: Kazimierz Wielki University, 2020-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<p>Attention of scientists of the world are study the features of increased resistance to hypoxia and stress, different development inflammation in them.</p><p><strong>The aim</strong> of the study was to trace the features of interleukins level in high- and low-resistance to hypoxic hypoxia rats (HR, LR) in different models of immobilization stress.</p><p><strong>Material and methods of </strong><strong>investigation</strong><strong>. </strong>The experiments were performed on 144 outbred HR and LR aged 5.5-6 months, dividing into 3 groups - control and 2 with different model of immobilizing stress. Determine concentration of interleukins 1beta, 2, 4, 6, 10 (IL-1beta, IL-2, IL-4, IL-6, IL-10), tumor necrosis factor alpha (TNF-alpha) in the blood serum.</p><p><strong>Results</strong>. High congenital resistance to hypoxia is associated with an increased content of anti-inflammatory cytokines (in males - IL-4 on 24.73% (p<0.001), in females - IL-10 on 37.36%; p<0.001). Intact males, compared with females, have a higher production of pro-inflammatory cytokines (IL-6, TNF-alpha). Intact males, compared with females, are dominated by the content of pro-inflammatory cytokines in the blood. In HR males, compared with females, a higher content of IL-6 (49.93%; p<0.01), TNF-alpha (10.85%; p<0.01), IL-4 (33.92%; p<0.001), lower - IL-1beta (37.91%; p<0.02) and IL-10 (93.06%; p<0.001). In LR males, compared with females, higher blood levels of IL-1beta (42.98%; p<0.001), IL-6 (54.32%; p<0.001), TNF-alpha (16,40%; p<0,002), the highest level of pro-inflammatory cytokines under stress with a frequency of every 24 hours. In HR males under these conditions, the level of IL-1beta increases (in 2.35 times; p<0.001), TNF-alpha (in 8.20 times; p<0.001). In exposed to stress every 72 hours in HR males, the level of TNF-alpha increases in 2.88 times (p<0.002), and in LR males in 5.18 times (p<0.001). At the same time, in HR females its level increases in 3.25 times (p<0.01), in LR females - in 5.73 times (p<0.01).</p><p><strong>Conclusion</strong>. The experiments revealed the changes of pro- and anti-inflammatory interleukins, which depends of sex, resistance to hypoxy and kinds of stress.</p>

Item Description

2391-8306
10.12775/JEHS.2020.10.05.030