Thin Layer Chromatographic Analysis of Beta-Lactam Antibiotics
Purpose: The paper describes some thin layer chromatographic procedures that allow simple and rapid separation and identification of penicillins and cephalosporins from complex mixtures. Methods: Using silicagel GF254 as stationary phase and selecting different mobile phases we succeeded in the sepa...
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| Main Authors: | , , , , , |
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| Format: | Book |
| Published: |
Tabriz University of Medical Sciences,
2013-08-01T00:00:00Z.
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| Online Access: | Connect to this object online. |
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| Summary: | Purpose: The paper describes some thin layer chromatographic procedures that allow simple and rapid separation and identification of penicillins and cephalosporins from complex mixtures. Methods: Using silicagel GF254 as stationary phase and selecting different mobile phases we succeeded in the separation of the studied beta-lactamins. Our aim was not only to develop a simple, rapid and efficient method for their separation but also the optimization of the analytical conditions. Results: No system will separate all the beta-lactams, but they could be identified when supplementary information is used from color reactions and/or by using additional chromatographic systems. Conclusions: The right combination of solvent system and detection method allows the identification of the studied penicillins and cephalosporins and can be successfully used in the preliminary analysis beta-lactam antibiotics. |
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| Item Description: | 10.5681/apb.2013.059 2228-5881 2251-7308 |