Phenotypic and Genotypic Characteristics of Antimicrobial Resistance in <i>Citrobacter freundii</i> Isolated from Domestic Ducks (<i>Anas platyrhynchos domesticus</i>) in Bangladesh

• Main Authors: Tarana Ahmed (Author), Md. Saiful Islam (Author), Najmul Haider (Author), Linzy Elton (Author), Badrul Hasan (Author), Mohammad Nuruzzaman (Author), Md. Tanvir Rahman (Author), S. M. Lutful Kabir (Author), Md. Shahidur Rahman Khan (Author)
• Format: Book
• Published: MDPI AG, 2023-04-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Antimicrobial resistance (AMR) in <i>Citrobacter freundii</i> poses a serious challenge as this species is one of the sources of nosocomial infection and causes diarrheal infections in humans. Ducks could be the potential source of multidrug-resistant (MDR) <i>C. freundii</i>; however, AMR profiles in <i>C. freundii</i> from non-human sources in Bangladesh have remained elusive. This study aimed to detect <i>C. freundii</i> in domestic ducks (<i>Anas platyrhynchos domesticus</i>) in Bangladesh and to determine their phenotypic and genotypic antibiotic susceptibility patterns. A total of 150 cloacal swabs of diseased domestic ducks were screened using culturing, staining, biochemical, polymerase chain reaction (PCR), and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) to detect <i>C. freundii</i>. Phenotypic and genotypic antibiotic susceptibility patterns were done by the disk diffusion method and PCR, respectively. In total, 16.67% (25/150) of the samples were positive for <i>C. freundii</i>. <i>C. freundii</i> isolates showed a range of 20% to 96% resistance to cefotaxime, gentamicin, levofloxacin, ciprofloxacin, cotrimoxazole, tetracycline, ampicillin, and cephalexin. More than 60% of the isolates were phenotypically MDR, and the index of multiple antibiotic resistance ranged from 0.07 to 0.79. Genes encoding resistance to beta-lactams [<i>bla</i>_TEM-1-88% (22/25), <i>bla</i>_CMY-2-56% (14/25), <i>bla</i>_CMY-9-8% (2/25), and <i>bla</i>_CTX-M-14-20% (5/25)], sulfonamides [<i>sul1</i>-52% (13/25), <i>sul2</i>-24% (6/25)], tetracyclines [<i>tetA</i>-32% (8/25) and <i>tetB</i>-4% (1/25)], aminoglycosides [<i>aacC4</i>-16% (4/25)], and fluoroquinolones [<i>qnrA</i>-4% (1/25), <i>qnrB</i>-12% (3/25), and <i>qnrS</i>-4% (1/25)] were detected in the isolated <i>C. freundii</i>. To the best of our knowledge, this is the first study in Bangladesh to detect MDR <i>C. freundii</i> with their associated resistance genes from duck samples. We suggest addressing the burden of diseases in ducks and humans and associated AMR issues using the One Health approach.

Item Description

10.3390/antibiotics12040769
2079-6382