Efficient Knockdown and Lack of Passenger Strand Activity by Dicer-Independent shRNAs Expressed from Pol II-Driven MicroRNA Scaffolds

• Main Authors: Erik Kaadt (Author), Sidsel Alsing (Author), Claudia R. Cecchi (Author), Christian Kroun Damgaard (Author), Thomas J. Corydon (Author), Lars Aagaard (Author)
• Format: Knjiga
• Izdano: Elsevier, 2019-03-01T00:00:00Z.
• Teme: article
• Online dostop: Connect to this object online.

The expression of short hairpin RNAs (shRNAs) may result in unwanted activity from the co-processed passenger strand. Recent studies have shown that shortening the stem of conventional shRNAs abolishes passenger strand release. These Dicer-independent shRNAs, expressed from RNA polymerase III (Pol I...