The Impact of Quercetin and Its Methylated Derivatives 3-o-Methylquercetin and Rhamnazin in Lipopolysaccharide-Induced Inflammation in Porcine Intestinal Cells

• Main Authors: Zita Karancsi (Author), Dóra Kovács (Author), Nikolett Palkovicsné Pézsa (Author), Péter Gálfi (Author), Ákos Jerzsele (Author), Orsolya Farkas (Author)
• Format: Book
• Published: MDPI AG, 2022-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Oxidative stress in the small intestine can lead to inflammation and barrier malfunction. The present study describes the effect of quercetin (Q), 3-o-methylquercetin (QM), and rhamnazin (R) on cell viability, paracellular permeability, production of intracellular reactive oxygen species (ROS), extracellular hydrogen peroxide (H₂O₂), and interleukin-6 (IL-6) after challenging jejunal cells (IPEC-J2) with different types (<i>Salmonella enterica</i> ser. Typhimurium, <i>Escherichia coli</i> O111:B4, and <i>E. coli</i> O127:B8) of lipopolysaccharides (LPS) applied in 10 µg/mL concentration. The intracellular ROS level increased after all LPS treatments, which could be decreased by all tested flavonoid compounds in 50 µM concentration. Extracellular H₂O₂ production significantly increased after Q and R treatment (50 µM). <i>S</i>. Typhimurium LPS could significantly increase IL-6 production of enterocytes, which could be alleviated by Q, QM, and R (50 µM) as well. Using fluorescein isothiocyanate dextran (FD4) tracer dye, we could demonstrate that <i>S</i>. Typhimurium LPS significantly increased the permeability of the cell layer. The simultaneous treatments of <i>S.</i> Typhimurium LPS and the flavonoid compounds showed no alteration in FD4 penetration compared to untreated cells. These results highlight that Q, QM, and R are promising substances that can be used to protect intestinal epithelial cells from the deteriorating effects of oxidative stress.

Item Description

10.3390/antiox11071265
2076-3921