Antifungal Agents Based on Chitosan Oligomers, ε-polylysine and <i>Streptomyces</i> spp. Secondary Metabolites against Three Botryosphaeriaceae Species

• Main Authors: Laura Buzón-Durán (Author), Jesús Martín-Gil (Author), Eduardo Pérez-Lebeña (Author), David Ruano-Rosa (Author), José L. Revuelta (Author), José Casanova-Gascón (Author), M. Carmen Ramos-Sánchez (Author), Pablo Martín-Ramos (Author)
• Format: Book
• Published: MDPI AG, 2019-07-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Grapevine trunk diseases (GTDs) are a major threat to the wine and grape industry. The aim of the study was to investigate the antifungal activity against <i>Neofusicoccum parvum</i>, <i>Diplodia seriata</i>, and <i>Botryosphaeria dothidea</i> of ε-polylysine, chitosan oligomers, their conjugates, <i>Streptomyces rochei</i> and <i>S. lavendofoliae</i> culture filtrates, and their binary mixtures with chitosan oligomers. In vitro mycelial growth inhibition tests suggest that the efficacy of these treatments, in particular those based on ε-polylysine and ε-polylysine:chitosan oligomers 1:1 <i>w</i>/<i>w</i> conjugate, against the three Botryosphaeriaceae species would be comparable to or higher than that of conventional synthetic fungicides. In the case of ε-polylysine, EC₉₀ values as low as 227, 26.9, and 22.5 µg·mL⁻¹ were obtained for <i>N. parvum</i>, <i>D. seriata</i>, and <i>B. dothidea</i>, respectively. Although the efficacy of the conjugate was slightly lower, with EC₉₀ values of 507.5, 580.2, and 497.4 µg·mL⁻¹, respectively, it may represent a more cost-effective option to the utilization of pure ε-polylysine. The proposed treatments may offer a viable and sustainable alternative for controlling GTDs.

Item Description

2079-6382
10.3390/antibiotics8030099