In Vivo Effect of a Nisin-Biogel on the Antimicrobial and Virulence Signatures of Canine Oral <i>Enterococci</i>

• Główni autorzy: Eva Cunha (Autor), Ana Filipa Ferreira (Autor), Sara Valente (Autor), Alice Matos (Autor), Luís Miguel Carreira (Autor), Marta Videira (Autor), Lélia Chambel (Autor), Luís Tavares (Autor), Manuela Oliveira (Autor)
• Format: Książka
• Wydane: MDPI AG, 2023-02-01T00:00:00Z.
• Hasła przedmiotowe: article
• Dostęp online: Connect to this object online.
• Notes: 10.3390/antibiotics12030468; 2079-6382
• Keywords: periodontal disease; dogs; nisin-biogel; <i>enterococci</i>; antimicrobial resistance; virulence signatures; Therapeutics. Pharmacology; RM1-950
• Source: Antibiotics, Vol 12, Iss 3, p 468 (2023)

Periodontal disease is a relevant oral disease in dogs and nisin-biogel has been previously proposed to be used in its control. <i>Enterococci</i>, as inhabitants of the oral cavity with a high genetic versatility, are a reliable bacterial model for antimicrobial studies. Our goal was to evaluate the in vivo influence of the long-term dental application of the nisin-biogel on the virulence and antimicrobial signatures of canine oral <i>enterococci</i>. Twenty dogs were randomly allocated to one of two groups (treatment group-TG with nisin-biogel dental application, or control group-CG without treatment) and submitted to dental plaque sampling at day 0 and after 90 days (T90). Samples were processed for <i>Enterococcus</i> spp. isolation, quantification, identification, molecular typing and antimicrobial and virulence characterization. From a total of 140 <i>enterococci</i>, molecular typing allowed us to obtain 70 representative isolates, mostly identified as <i>E. faecalis</i> and <i>E. faecium</i>. No significant differences (<i>p</i> > 0.05) were observed in the virulence index of the isolates obtained from samples collected from the TG and CG at T90. At T90, a statistically significant difference (<i>p</i> = 0.0008) was observed in the antimicrobial resistance index between the isolates from the TC and CG. Oral <i>enterococci</i> were revealed to be reservoirs of high resistant and virulent phenotypes.