Quantitative analysis of mRNA translation in mammalian spermatogenic cells with sucrose and Nycodenz gradients

• Main Authors: Bagarova Jana (Author), Kleene Kenneth C (Author), Hawthorne Sabrina K (Author), Catado Leah M (Author)
• Format: Book
• Published: BMC, 2010-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

<p>Abstract</p> <p>Background</p> <p>Developmental and global regulation of mRNA translation plays a major role in regulating gene expression in mammalian spermatogenic cells. Sucrose gradients are widely used to analyze mRNA translation. Unfortunately, the information...