Antimicrobial and antioxidant activities of the extracts and compounds from the leaves of <it>Psorospermum aurantiacum</it> Engl. and <it>Hypericum lanceolatum</it> Lam.
<p>Abstract</p> <p>Background</p> <p><it>Psorospermun aurantiacum</it> and <it>Hypericum lanceolatum</it> are plants locally used in Cameroon and other parts of Africa for the treatment of gastrointestinal and urinary tract infections, skin infec...
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| Format: | Book |
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BMC,
2012-08-01T00:00:00Z.
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| Summary: | <p>Abstract</p> <p>Background</p> <p><it>Psorospermun aurantiacum</it> and <it>Hypericum lanceolatum</it> are plants locally used in Cameroon and other parts of Africa for the treatment of gastrointestinal and urinary tract infections, skin infections, venereal diseases, gastrointestinal disorder, infertility, epilepsy as well as microbial infections. The present study was designed in order to investigate the <it>in vitro</it> antimicrobial and radical scavenging activities of the extracts and isolated compounds from the leaves of these plants.</p> <p>Methods</p> <p>The plant extract was prepared by maceration in ethyl acetate and methanol and fractionated by column chromatography. The structures of isolated compounds were elucidated by spectroscopic analyses in conjunction with literature data. The broth microdilution method was used to evaluate the <it>in vitro</it> antimicrobial activity against bacteria, yeasts and dermatophytes. The antioxidant potentials of the extracts and their isolated compounds were evaluated using the DPPH radical scavenging method.</p> <p>Results</p> <p>Five known compounds: physcion (<b>1</b>), 1,8-dihydroxy-3-geranyloxy-6-methylanthraquinone (<b>2</b>), kenganthranol B (<b>3</b>), vismiaquinone (<b>4</b>), and octacosanol (<b>5</b>) were isolated from the leaves of <it>P</it>. <it>aurantiacum</it> while six compounds including friedelin (<b>6</b>), betulinic acid (<b>7</b>), 2,2',5,6'-tetrahydroxybenzophenone (<b>8</b>), allanxanthone A (<b>9</b>), 1,3,6- trihydroxyxanthone (<b>10</b>) and isogarcinol (<b>11</b>) were isolated from <it>H. lanceolatum.</it> Compound 8 and 4 exhibited the highest antibacterial and antifungal activities with MIC ranges of 2-8 μg/ml and 4-32 μg/ml respectively. <it>P. aurantiacum</it> crude extract (Rsa50 = 6.359 ± 0.101) showed greater radical scavenging activity compared with <it>H. lanceolatum</it> extract (Rsa50 = 30.996 ± 0.879). Compound <b>11</b> showed the highest radical scavenging activity (RSa<sub>50</sub> = 1.012 ± 0.247) among the isolated compounds, comparable to that of L-arscobic acid (RSa50 = 0.0809 ± 0.045).</p> <p>Conclusions</p> <p>The experimental findings show that the ethyl acetate and methanol extracts and isolated compounds from <it>P. aurantiacum</it> and <it>H. lanceolatum</it> stem bark possess significant antimicrobial and antioxidant activities justifying the use of these plants in traditional medicine, which may be developed as phytomedicines.</p> |
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| Item Description: | 10.1186/1472-6882-12-136 1472-6882 |