Hydrogen Sulfide Relaxes Human Uterine Artery via Activating Smooth Muscle BK_Ca Channels

• Main Authors: Yan Li (Author), Jin Bai (Author), Yi-hua Yang (Author), Naoto Hoshi (Author), Dong-bao Chen (Author)
• Format: Book
• Published: MDPI AG, 2020-11-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.3390/antiox9111127; 2076-3921
• Keywords: hydrogen sulfide; BK_Ca channels; smooth muscle; uterine artery; women; Therapeutics. Pharmacology; RM1-950
• Source: Antioxidants, Vol 9, Iss 11, p 1127 (2020)

Opening of large conductance calcium-activated and voltage-dependent potassium (BK_Ca) channels hyperpolarizes plasma membranes of smooth muscle (SM) to cause vasodilation, underling a key mechanism for mediating uterine artery (UA) dilation in pregnancy. Hydrogen sulfide (H₂S) has been recently identified as a new UA vasodilator, yet the mechanism underlying H₂S-induced UA dilation is unknown. Here, we tested whether H₂S activated BK_Ca channels in human UA smooth muscle cells (hUASMC) to mediate UA relaxation. Multiple BK_Ca subunits were found in human UA in vitro and hUASMC in vitro, and high β1 and γ1 proteins were localized in SM cells in human UA. Baseline outward currents, recorded by whole-cell and single-channel patch clamps, were significantly inhibited by specific BK_Ca blockers iberiotoxin (IBTX) or tetraethylammonium, showing specific BK_Ca activity in hUASMC. H₂S dose (NaHS, 1-1000 µM)-dependently potentiated BK_Ca currents and open probability. Co-incubation with a Ca²⁺ blocker nifedipine (5 µM) or a chelator (ethylene glycol-bis (β-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA), 5 mM) did not alter H₂S-potentiated BK_Ca currents and open probability. NaHS also dose-dependently relaxed phenylephrine pre-constricted freshly prepared human UA rings, which was inhibited by IBTX. Thus, H₂S stimulated human UA relaxation at least partially via activating SM BK_Ca channels independent of extracellular Ca²⁺.