Reliability of CNEURO hyssops for sample collection in the SARS-CoV-2 diagnosis

• Main Authors: Cristobal Gonzáles-Losada (Author), Luis Gabriel González-Lodeiro (Author), Abraham Ismael Beato Canfux (Author), Julio Raúl Fernández (Author), Hamlet Camacho (Author), Diana Vázquez-Blomquist (Author), Gerardo Enrique Guillén-Nieto (Author)
• Format: Book
• Published: Universidad de Ciencias Médicas de La Habana, 2022-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 1729-519X
• Keywords: covid-19, sars-cov-2 diagnosis, nasopharyngeal swab, oropharyngeal swab; Medicine (General); R5-920; Public aspects of medicine; RA1-1270
• Source: Revista Habanera de Ciencias Médicas, Vol 21, Iss 1, Pp e4512-e4512 (2022)

Introduction: The detection of SARS-CoV-2 genetic material from nasopharyngeal swab samples by RT-PCR is the most specific and sensitive way to test suspected cases. However, factors such as the sampling process, the type of hyssop used, and the anatomical area from which the sample is collected can distort the result and cause false negatives. Objective: To evaluate the reliability of CNUERO hyssops for sample collection for the SARS-CoV-2 diagnosis versus IMPROSWAB hyssops. Material and Methods: To study the reliability of hyssops developed in Cuba for swabbing for the COVID-19 diagnosis by comparing them to other hyssops successfully used for this task, 2 swabbing samples were obtained from each patient (136). One of these two samples was taken using the hyssops made in Cuba, while the other was taken using another hyssop imported from Germany. The positive detections obtained with the use of both hyssops were compared using the Fisher' s exact test. The result of the detection of each hyssop was evaluated and compared using the ROC curve. Results: The use of CNEURO hyssops allowed the detection of 45 out of 59 positive cases, while IMPROSAWAB hyssops detected 52 out of 59 true positive cases. There were no significant differences between positive cases detected with the use of each hyssop. The sensitivity of sample detection using CNEURO hyssops was 76,3 % while the one using IMPROSWAB hyssops was 88,1 %. Hence, there are no significant differences in the detection of cases using these two hyssops. Conclusion: CNEURO hyssops are safe and reliable to be used to take nasopharyngeal samples from COVID-19 patients.