HPLC-PDA-ESI-MS/MS Profiling and Anti-Biofilm Potential of <i>Eucalyptus</i><i>sideroxylon</i> Flowers

• Main Authors: Mona M. Okba (Author), Riham A. El-Shiekh (Author), Mohammed Abu-Elghait (Author), Mansour Sobeh (Author), Rehab M. S. Ashour (Author)
• Format: Book
• Published: MDPI AG, 2021-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

The development of multidrug-resistant bacterial strains is a worldwide emerging problem that needs a global solution. Exploring new natural antibiofilm agents is one of the most important alternative therapies in combating bacterial infections. This study aimed at testing the antimicrobial potential of <i>Eucalyptus sideroxylon</i> flowers extract (ESFE) against <i>Bacillus subtilis</i>, <i>Staphylococcus aureus</i>, <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i> and <i>Candida albicans</i> prior to testing the antibiofilm activity against <i>S. aureus</i>, <i>P. aeruginosa</i> and <i>C. albicans</i>. ESFE demonstrated antimicrobial activity and promising inhibition activity against methicillin-resistant <i>S. aureus</i> (MRSA) biofilm formation up to 95.9% (<i>p</i> < 0.05) at a concentration of 0.05 mg/mL and eradicated <i>C. albicans</i> biofilm formation up to 71.2% (<i>p</i> < 0.05) at a concentration of 0.7 mg/mL. LC-MS analysis allowed the tentative identification of eighty-three secondary metabolites: 21 phloroglucinol, 18 terpenes, 16 flavonoids, 7 oleuropeic acid derivatives, 7 ellagic acid derivatives, 6 gallic acid derivatives, 3 phenolic acids, 3 fatty acids and 2 miscellaneous. In conclusion, <i>E. sideroxylon</i> is a rich source of effective constituents that promote its valorization as a promising candidate in the management of multidrug-resistant bacterial infections.

Item Description

10.3390/antibiotics10070761
2079-6382