GINGIVAL FIBROBLASTS AND NAK-1-ACTIVATED PRO-B LYMPHOCYTES APOPTOSIS

• Main Authors: Carmen Gentimir (Amititeloaie) (Author), Anda Cătălina Zaharia (Author), Eugen Bitere (Author), Dragoş Acatrinei (Author), Ionuţ Cazan (Author), Marcel Costuleanu (Author), Otilia Boişteanu (Author)
• Format: Book
• Published: Romanian Society of Oral Rehabilitation, 2020-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 2066-7000; 2601-4661
• Keywords: apoptosis; autophagy; gingival fibroblast; nak-1; pro-b lymphocyte; Dentistry; RK1-715
• Source: Romanian Journal of Oral Rehabilitation, Vol 12, Iss 4, Pp 51-56 (2020)

Aim of the study We studied the effects of fibroblasts autophagy on the apoptosis of pro-B murine lymphocytes when were co-cultured. Material and methods Study of literature suggested that released autophagy-derived metabolites from fibroblasts might affect the apoptotic behavior of lymphocytes when there is a close interaction of these cells. The experimental plan of the actual studies was represented by induced effects of the presence of gingival fibroblasts in co-culture on NAK-1 receptors-activated pro-lymphocytes B apoptosis. Results We clearly showed that murine pro-B lymphocyte apoptosis induced by indol-3-carbinol could be significantly enhanced by the presence of gingival fibroblasts in co-culture. Furthermore, the addition of 3-methyl-adenine in the medium for 24 hours blocked the enhancing effects induced by fibroblasts on the apoptotic index of pro-B lymphocytes. Conclusions So, the enhancing apoptotic effects of fibroblasts on pro-B lymphocytes are related to released autophagy-derived metabolites. Their real nature remains to be established through future researches involving this complex system.