A combined treatment with progesterone, anti-inhibin serum, and equine chorionic gonadotropin improves number of ovulated oocytes in young C57BL/6J mice
Superovulation procedures are routinely and widely used in mouse reproductive technology. Previous studies have shown that a large number of oocytes can be obtained from adult mice (> 10 weeks old) using a combined treatment with progesterone (P4) and anti-inhibin serum (AIS). However, these effe...
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The Society for Reproduction and Development,
2023-06-01T00:00:00Z.
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| LEADER | 00000 am a22000003u 4500 | ||
|---|---|---|---|
| 001 | doaj_722a0cab955d43fdb5993997ba3eeb51 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Atsuko KAGEYAMA |e author |
| 700 | 1 | 0 | |a Mizuho TSUCHIYA |e author |
| 700 | 1 | 0 | |a Jumpei TERAKAWA |e author |
| 700 | 1 | 0 | |a Junya ITO |e author |
| 700 | 1 | 0 | |a Naomi KASHIWAZAKI |e author |
| 245 | 0 | 0 | |a A combined treatment with progesterone, anti-inhibin serum, and equine chorionic gonadotropin improves number of ovulated oocytes in young C57BL/6J mice |
| 260 | |b The Society for Reproduction and Development, |c 2023-06-01T00:00:00Z. | ||
| 500 | |a 0916-8818 | ||
| 500 | |a 1348-4400 | ||
| 500 | |a 10.1262/jrd.2023-036 | ||
| 520 | |a Superovulation procedures are routinely and widely used in mouse reproductive technology. Previous studies have shown that a large number of oocytes can be obtained from adult mice (> 10 weeks old) using a combined treatment with progesterone (P4) and anti-inhibin serum (AIS). However, these effects have not been fully investigated in young (4 weeks) C57BL/6J mice. Here, we found that a modified superovulation protocol (combined treatment with P4, AIS, eCG (equine chorionic gonadotropin), and hCG (human chorionic gonadotropin); P4D2-Ae-h) improved the number of oocytes compared to the control (eCG and hCG) (39.7 vs. 21.3 oocytes/mouse). After in vitro fertilization, pronuclear formation rates were 69.3% (P4D2-Ae-h group) and 66.2% (control group). After embryo transfer, 46.4% (116/250) of the embryos in the P4D2-Ae-h group successfully developed to term, which was comparable to the control group (42.9%; 123/287 embryos). In conclusion, our protocol (P4D2-Ae-h) was effective for superovulation in young C57BL/6J mice. | ||
| 546 | |a EN | ||
| 690 | |a anti-inhibin serum | ||
| 690 | |a ecg | ||
| 690 | |a progesterone | ||
| 690 | |a reproductive engineering | ||
| 690 | |a superovulation | ||
| 690 | |a Reproduction | ||
| 690 | |a QH471-489 | ||
| 690 | |a Internal medicine | ||
| 690 | |a RC31-1245 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n The Journal of Reproduction and Development, Vol 69, Iss 4, Pp 223-226 (2023) | |
| 787 | 0 | |n https://www.jstage.jst.go.jp/article/jrd/69/4/69_2023-036/_pdf/-char/en | |
| 787 | 0 | |n https://doaj.org/toc/0916-8818 | |
| 787 | 0 | |n https://doaj.org/toc/1348-4400 | |
| 856 | 4 | 1 | |u https://doaj.org/article/722a0cab955d43fdb5993997ba3eeb51 |z Connect to this object online. |