Common polymorphisms of calpain-10 and the risk of Type 2 Diabetes in a Tunisian Arab population: a case-control study

• Main Authors: Mahjoub Touhami (Author), Al-Khateeb Ghada M (Author), Kacem Maha (Author), Chaieb Molka (Author), Messaoudi Safia (Author), Turki Amira (Author), Ezzidi Intissar (Author), Almawi Wassim Y (Author), Mtiraoui Nabil (Author)
• Format: Book
• Published: BMC, 2010-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.1186/1471-2350-11-75; 1471-2350
• Keywords: Internal medicine; RC31-1245; Genetics; QH426-470
• Source: BMC Medical Genetics, Vol 11, Iss 1, p 75 (2010)

<p>Abstract</p> <p>Background</p> <p>Genetic variations in the calpain-10 gene (<it>CAPN10</it>), in particular the at-risk diplotype (112/121), were previously implicated with increased risk of type 2 diabetes (T2D).</p> <p>Methods</p> <p>We examined the association of <it>CAPN10 </it>UCSNP-43 (rs3792267), UCSNP-19 (rs3842570), and UCSNP-63 (rs5030952) SNPs with T2D in 917 Tunisian T2D patients and 748 non-diabetic controls. <it>CAPN10 </it>genotyping was done by PCR-RFLP.</p> <p>Results</p> <p>Enrichment of UCSNP-19 2R (minor) allele and 2R/2R genotype was found in T2D patients; the allele and genotype distribution of UCSNP-43 and UCSNP-63 alleles and genotypes were not significantly different between patient groups and non-diabetic control subjects. Regression analysis demonstrated progressive increases in T2D risk in 3R/2R [OR (95% CI) = 1.35 (1.08 - 1.68)] and 2R/2R [OR (95% CI) = 1.61 (1.20 - 2.18)] genotypes. Of the six haplotypes detected, enrichment of haplotype 111 (UCSNP-43/UCSNP-19/UCSNP-63) was seen in patients (<it>Pc </it>= 0.034); the distribution of the other haplotypes was comparable between patients and control subjects; neither haplotype 211 nor haplotype 212 was observed. Furthermore, the frequency of all <it>CAPN10 </it>diplotypes identified, including the "high-risk diplotype (112/121) reported for Mexican-Americans and Northern Europeans, were comparable between patients and controls.</p> <p>Conclusions</p> <p><it>CAPN10 </it>UCSNP-19 variant, and the 111 haplotype contribute to the risk of T2D in Tunisian subjects; no significant associations between <it>CAPN10 </it>diplotypes and T2D were demonstrated for Tunisians.</p>