Research on Correlations of miR-196a Expression with Progression and Prognosis of Cutaneous Squamous Cell Carcinoma

• Main Authors: Zhang W (Author), Yang X (Author), Lv J (Author), An S (Author)
• Format: Book
• Published: Dove Medical Press, 2022-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Wei Zhang,1 Xiaowen Yang,2 Jianping Lv,1 Shichang An3 1Department of Cosmetic Plastic Surgery, Weifang People's Hospital, Weifang, Shandong, 261000, People's Republic of China; 2Department of Stomatology, The People's Hospital of Zhaoyuan City, Yantai, Shandong, 265400, People's Republic of China; 3Department of Oral and Maxillofacial Surgery, Weifang People's Hospital, Weifang, Shandong, 261000, People's Republic of ChinaCorrespondence: Shichang AnDepartment of Oral and Maxillofacial Surgery, Weifang People's Hospital, 151 Guangwen Street, Weifang, Shandong, 261000, People's Republic of China, Tel [phone], Email [e-mail]: This study aimed to investigate the correlation between miR-196a expression and the progression and prognosis of cutaneous squamous cell carcinoma (CSCC).Patients and Methods: Tissue samples and corresponding paracancerous tissue samples from 117 patients with CSCC were collected. The qRT-PCR analysis was used to detect the expression levels of miR-196a. Kaplan-Meier curve and Cox regression analyses were used to analyze the relationship between miR-196a expression and patients' prognosis. The CCK-8 and transwell assays were used to explore the effects of miR-196a on the abilities of cell proliferation, migration, or invasion.Results: miR-196a expression was significantly up-regulated in CSCC tissues or cell lines, compared with adjacent normal tissues or cell lines, respectively. High expression of miR-196a was associated with positive lymph node metastasis, high TNM stages, and a lower five-year survival rate. The expression level of miR-196a was up-regulated and the proliferation, migration or invasion ability of cells were significantly increased accordingly.Conclusion: miR-196a is highly expressed in CSCC, thus affecting the occurrence and development of CSCC. More importantly, miR-196a was shown to have potential as a prognostic marker for CSCC.Keywords: miR-196a, progression, prognosis, cutaneous squamous cell carcinoma

Item Description

1178-7015