COMPARACIÓN DE LA PRUEBA TRIDIMENSIONAL CON PCR MÚLTIPLE, PARA DETECCIÓN DE CARBAPENEMASAS
Objective: To compare the phenotypic test for detection of carbapenemases (Three-dimensional Test - THT), with molecular biology test (Multiplex PCR), for the detection of resistance genes. Methods: A total of 118 isolates of Gram-negative bacteria strains from the multiresistant surveillance progra...
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| Format: | Book |
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Asociación Colombiana de Infectología,
2018-09-01T00:00:00Z.
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| Summary: | Objective: To compare the phenotypic test for detection of carbapenemases (Three-dimensional Test - THT), with molecular biology test (Multiplex PCR), for the detection of resistance genes. Methods: A total of 118 isolates of Gram-negative bacteria strains from the multiresistant surveillance program at a third-level hospital were evaluated for the detection of carbapenemases by three-dimensional test (THT) and the molecular biology PCR Multiple method . We calculated sensitivity, specificity, PPV, NPV, validity index and Youden index (IY), with their confidence intervals. Results: THT test compared to the multiple PCR test had a sensitivity of 98.41% (95% CI 94.53 - 100), specificity was 83.64% (95% CI 72.95 - 94 , 32) and positive and negative predictive values were respectively 87.32 (95% CI 78.88 - 95.77) and 97.87 (95% CI 92.68 - 100). Conclusion: The Hodge three-dimensional test (THT), for detection of carbapenem resistance, may be a useful routine laboratory test to suggest resistance by carbapenemases |
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| Item Description: | 10.22354/in.v22i4 0123-9392 0123-9392 |