Protective Effect of the Hexanic Extract of <i>Eryngium carlinae</i> Inflorescences In Vitro, in Yeast, and in Streptozotocin-Induced Diabetic Male Rats

• Main Authors: Donovan J. Peña-Montes (Author), Maribel Huerta-Cervantes (Author), Mónica Ríos-Silva (Author), Xóchitl Trujillo (Author), Miguel Huerta (Author), Ruth Noriega-Cisneros (Author), Rafael Salgado-Garciglia (Author), Alfredo Saavedra-Molina (Author)
• Format: Book
• Published: MDPI AG, 2019-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

In the present study, we investigated the composition and antioxidant activity of the hexanic extract of <i>Eryngium carlinae</i> inflorescences by employing in vitro assays to measure antioxidant capacity and 2,2-diphenyl-1-picrylhydrazyl scavenging activity. We also applied the hexanic extract to <i>Saccharomyces cerevisiae</i>, under hydrogen peroxide-induced stress. Finally, we tested the extract in male Wistar rats with and without streptozotocin-induced diabetes. The compounds in the hexanic extract were analyzed by gas-chromatography-mass spectrometry, which revealed mainly terpenes and sesquiterpenes, including (Z)β-farnesene (38.79%), β-pinene (17.53%), calamene (13.3%), and α-farnesene (10.38%). In vitro and in <i>S. cerevisiae</i>, the extract possessed antioxidant activity at different concentrations, compared to ascorbic acid (positive control). In normoglycemic and hyperglycemic rats, oral administration of 30 mg/kg of the extract reduced blood glucose levels; lipid peroxidation in liver, kidney and brain; protein carbonylation; and reactive oxygen species (ROS) production. It also increased catalase activity in the brain, kidneys and liver. These findings show that this hexanic extract of <i>E. carlinae</i> inflorescences possessed antioxidant properties.

Item Description

2076-3921
10.3390/antiox8030073