Antimicrobial activity of different concentrations of NaOCl and chlorhexidine using a contact test

• Main Authors: Sassone Luciana Moura (Author), Fidel Rivail Antonio Sergio (Author), Fidel Sandra Rivera (Author), Dias Marina (Author), Hirata Junior Raphael (Author)
• Format: Book
• Published: Fundação Odontológica de Ribeirão Preto, 2003-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 0103-6440
• Keywords: antimicrobial activity; sodium hypochlorite; chlorhexidine; endodontics; Dentistry; RK1-715
• Source: Brazilian Dental Journal, Vol 14, Iss 2, Pp 99-102 (2003)

The purpose of this study was to analyze the in vitro antimicrobial activity of sodium hypochlorite (1% and 5%) and chlorhexidine (0.12%, 0.5% and 1%). Bacterial samples (ATCC) of Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Porphyromonas gingivalis and Fusobacterium nucleatum were submitted to a contact test. Solutions were evaluated at different time intervals: immediately, 5 min, 15 min, and 30 min after contact and repeated 10 times. The results of the contact test showed that 0.12% chlorhexidine did not eliminate E. faecalis at any time interval, while 0.5% and 1% chlorhexidine and 1% and 5% sodium hypochlorite did. These results permit us to conclude that to obtain better antimicrobial activity, chlorhexidine in a concentration greater than 0.12% should be used.