Development of a Reporter System to Explore MMEJ in the Context of Replacing Large Genomic Fragments

• Main Authors: Mert Yanik (Author), Surya Prakash Goud Ponnam (Author), Tobias Wimmer (Author), Lennart Trimborn (Author), Carina Müller (Author), Isabel Gambert (Author), Johanna Ginsberg (Author), Annabella Janise (Author), Janina Domicke (Author), Wolfgang Wende (Author), Birgit Lorenz (Author), Knut Stieger (Author)
• Format: Book
• Published: Elsevier, 2018-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Common genome-editing strategies are either based on non-homologous end joining (NHEJ) or, in the presence of a template DNA, based on homologous recombination with long (homology-directed repair [HDR]) or short (microhomology-mediated end joining [MMEJ]) homologous sequences. In the current study,...