The effect of watermelon frost on prostaglandin E₂ (PGE₂) in inflamed pulp tissue (in vitro study)

• Main Authors: Dennis Dennis (Author), Trimurni Abidin (Author)
• Format: Book
• Published: Universitas Airlangga, 2009-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Background: </strong><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Pulp inflammation can be marked by the increase of prostaglandin E</em><span style="font-family: TribuneItalic; font-size: 7pt; color: #231f20; font-style: normal; font-variant: normal;"><em>2</em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>(PGE</em><span style="font-family: TribuneItalic; font-size: 7pt; color: #231f20; font-style: normal; font-variant: normal;"><em>2</em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>) level compared to normal pulp. The </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>increase of PGE</em><span style="font-family: TribuneItalic; font-size: 7pt; color: #231f20; font-style: normal; font-variant: normal;"><em>2 </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>may lead to vasodilatation, increase of vascular permeability, pain and bone resorption. Watermelon frost has been </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>well known in Chinese society for pain relief and inflammation in oral cavity and teeth. </em><span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Purpose: </strong><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The aim of this study was to investigate </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>that watermelon frost can be used to decrease the PGE</em><span style="font-family: TribuneItalic; font-size: 7pt; color: #231f20; font-style: normal; font-variant: normal;"><em>2 </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>level. </em><span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Method: </strong><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>27 samples of pulp tissues used in this in-vitro study, were </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>extirpated from the patients' teeth with symptomatic irreversible pulpitis referred to clinic of Conservative Dentistry, RSPGM Faculty </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>of Dentistry, USU. Trial materials were applied to 27 samples i.e. watermelon frost as a trial material and commercial watermelon </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>frost and eugenol to observe their effect on PGE</em><span style="font-family: TribuneItalic; font-size: 7pt; color: #231f20; font-style: normal; font-variant: normal;"><em>2</em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>. PGE</em><span style="font-family: TribuneItalic; font-size: 7pt; color: #231f20; font-style: normal; font-variant: normal;"><em>2 </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>level of each material was detected through ELISA method by measuring </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>and comparing the absorbance reading of the wells of the samples against standards with a micro plate reader at W</em><span style="font-family: TribuneItalic; font-size: 7pt; color: #231f20; font-style: normal; font-variant: normal;"><em>1 </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>= 650 nm and </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>W</em><span style="font-family: TribuneItalic; font-size: 7pt; color: #231f20; font-style: normal; font-variant: normal;"><em>2 </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>= 490 nm. </em><span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Result: </strong><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The result showed the biggest effect was found in the third group (eugenol), mean 4.6933, followed by the first </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>group (watermelon frost as a trial material), mean 18,1578 then the second group (commercial watermelon frost), mean 82,2689. OneWay ANOVA revealed that there were significant differences among all trial materials (p < 0.001) on PGE</em><span style="font-family: TribuneItalic; font-size: 7pt; color: #231f20; font-style: normal; font-variant: normal;"><em>2 </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>level</em><span style="font-family: TribuneItalic; font-size: 7pt; color: #231f20; font-style: normal; font-variant: normal;"><em>. </em><span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Conclusion: </strong><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>This </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>study demonstrated that watermelon frost can be used to decrease the PGE</em><span style="font-family: TribuneItalic; font-size: 7pt; color: #231f20; font-style: normal; font-variant: normal;"><em>2 </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>level in inflamed pulp tissue and led to the acceptance of </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>traditional medicine and natural products as an alternative form of dental care.</em></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>

Item Description

1978-3728
2442-9740
10.20473/j.djmkg.v42.i2.p82-85