Measurement of Oxidative Stress Index in Seminal Plasma Can Predict In Vivo Fertility of Liquid-Stored Porcine Artificial Insemination Semen Doses

• Main Authors: Isabel Barranco (Author), Camila P. Rubio (Author), Asta Tvarijonaviciute (Author), Heriberto Rodriguez-Martinez (Author), Jordi Roca (Author)
• Format: Book
• Published: MDPI AG, 2021-07-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

The study evaluated the relation between the oxidative stress index (OSI) in porcine seminal plasma (<i>n</i> = 76) with sperm resilience and in vivo fertility (farrowing rate and litter size of 3137 inseminated sows) of liquid-stored artificial insemination (AI) semen doses. The OSI was assessed as the ratio of advanced oxidation protein products to Trolox-equivalent antioxidant capacity, both measured using an automated analyzer. Sperm motility (computer-assisted sperm analyzer) and viability (flow cytometry) were evaluated in semen AI-doses at 0 and 72 h of storage at 17 °C. Sperm resilience was defined as the difference between storage intervals. Semen AI-doses were hierarchically clustered as having high, medium and low seminal OSI (<i>p</i> < 0.001) with those of low displaying higher resilience (<i>p</i> < 0.01). Boars were hierarchically clustered into two groups (<i>p</i> < 0.001) as having either positive or negative farrowing rate and litter size deviation; the negative one showing higher seminal OSI (<i>p</i> < 0.05). In sum, seminal OSI was negatively related to sperm motility and the in vivo fertility of liquid-stored boar semen AI-doses, with the receiver operating characteristic curve presenting seminal OSI as a good predictive biomarker of in vivo fertility of AI-boars (area under the curve: 0.815, <i>p</i> < 0.05).

Item Description

10.3390/antiox10081203
2076-3921