Chemical composition and antibacterial activity of Origanum ramonense essential oil on the β-lactamase and extended- spectrum β-lactamase urinary tract isolates
The aim of this study was to evaluate the antibacterial activity of Origanum ramonense essential oil extracted from the air-dried leaves against β-lactamase and extended-spectrum β-lactamase obtained from the patients with urinary tract infection. The essential oil was extracted by hydrodistillation...
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Bangladesh Pharmacological Society,
2018-09-01T00:00:00Z.
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| Summary: | The aim of this study was to evaluate the antibacterial activity of Origanum ramonense essential oil extracted from the air-dried leaves against β-lactamase and extended-spectrum β-lactamase obtained from the patients with urinary tract infection. The essential oil was extracted by hydrodistillation and analyzed by GC-MS. In vitro antibacterial activity was studied using disc diffusion and microdilution methods. Twenty compounds were identified representing 97.8% of the total oil. The major components were carvacrol (84.6%), p-cymene (4.3%) and γ-terpinene (3.3%). The oil showed a broad spectrum of antibacterial activity against all tested isolates. Staphylococcus aureus, S. epidermidis, Klebsiella pneumoniae and Enterobacter aerogenes had the lowest minimum inhibitory concentration values (0.015 µg/mL) followed by Escherichia coli (0.14 µg/mL). The lowest susceptible strains to oil were Pseudomonas aeruginosa, Proteus mirabilis, E. coli 25922 and P. aeruginosa 10145. The bacteriostatic and bactericidal effects at concentrations as low as 0.015 µg/mL indicated the potent antibacterial activity of O. ramonense. Video Clip of Methodology: Disc Diffusion method: 2 min 16 sec Full Screen Alternate |
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| Item Description: | 10.3329/bjp.v13i3.36897 1991-0088 |