Genetic Diversity of Virulent Polymyxin-Resistant <i>Klebsiella aerogenes</i> Isolated from Intensive Care Units
This study evaluated the scope and genetic basis of polymyxin-resistant <i>Klebsiella aerogenes</i> in Brazil. Eight polymyxin-resistant and carbapenemase-producing <i>K. aerogenes</i> strains were isolated from patients admitted to the ICU of a tertiary hospital. Bacterial s...
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| Main Authors: | , , , , , , |
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| Format: | Book |
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MDPI AG,
2022-08-01T00:00:00Z.
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| Summary: | This study evaluated the scope and genetic basis of polymyxin-resistant <i>Klebsiella aerogenes</i> in Brazil. Eight polymyxin-resistant and carbapenemase-producing <i>K. aerogenes</i> strains were isolated from patients admitted to the ICU of a tertiary hospital. Bacterial species were identified by automated systems and antimicrobial susceptibility profile was confirmed using broth microdilution. The strains displayed a multidrug resistant profile and were subjected to whole-genome sequencing. Bioinformatic analysis revealed a variety of antimicrobial resistance genes, including the <i>bla</i><sub>KPC-2</sub>. No plasmid-mediated colistin resistance gene was identified. Nonetheless, nonsynonymous mutations in <i>mgrB, pmrA, pmrB,</i> and <i>eptA</i> were detected, justifying the colistin resistance phenotype. Virulence genes encoding yersiniabactin, colibactin, and aerobactin were also found, associated with ICE<i>Kp4</i> and ICE<i>Kp10</i>, and might be related to the high mortality observed among the patients. In fact, this is the first time ICE<i>Kp</i> is identified in <i>K. aerogenes</i> in Brazil. Phylogenetic analysis grouped the strains into two clonal groups, belonging to ST93 and ST16. In summary, the co-existence of antimicrobial resistance and virulence factors is deeply worrying, as it could lead to the emergence of untreatable invasive infections. All these factors reinforce the need for surveillance programs to monitor the evolution and dissemination of multidrug resistant and virulent strains among critically ill patients. |
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| Item Description: | 10.3390/antibiotics11081127 2079-6382 |