Neuroprotective Effects of <i>Pulicaria incisa</i> Infusion on Human Neuroblastoma Cells and Hippocampal Neurons

• Main Authors: Talya Barak (Author), Oshrat Miller (Author), Sarit Melamed (Author), Zipora Tietel (Author), Moti Harari (Author), Eduard Belausov (Author), Anat Elmann (Author)
• Format: Book
• Published: MDPI AG, 2022-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Reactive oxygen species (ROS) and oxidative stress increase susceptibility to neurodegeneration and other age-related pathologies. We have previously demonstrated that an infusion prepared from <i>Pulicaria incisa</i> (<i>Pi</i>) has protective, anti-inflammatory, and antioxidative effects in glial cells. However, the neuroprotective activities of <i>Pi</i> infusion in cultured neurons and aging mice have never been studied. In the following study, the effects of <i>Pi</i> infusion were explored in a hydrogen peroxide (H₂O₂)-induced oxidative stress model in SH-SY5Y human neuroblastoma cells. Profiling of the infusion by gas chromatography-mass spectrometry identified chlorogenic acid, quercetin, and aucubin as some of its main constituents. H₂O₂-induced ROS accumulation and caspase 3 activity decreased SH-SY5Y viability and were prevented upon the pretreatment of cells with <i>Pi</i> infusion. Additionally, the <i>Pi</i> infusion upregulated cellular levels and the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) as well as the phosphorylation of cyclic AMP response element-binding protein (CREB). Aging mice treated daily for 18 months with <i>Pi</i> infusion exhibited reduced neuronal cell death in the hippocampus as compared to age-matched controls. We, therefore, propose <i>Pi</i> infusion as a candidate regulator of oxidative stress in the brain.

Item Description

10.3390/antiox12010032
2076-3921