IS4‐FAM, a fluorescent tool to study CXCR4 affinity and competitive antagonism in native cancer cells

• Main Authors: Isabel Hamshaw (Author), Marco M. D. Cominetti (Author), Princess Nana‐Akyin (Author), Ernie Ho Yee Ho (Author), Mark Searcey (Author), Anja Mueller (Author)
• Format: Book
• Published: Wiley, 2024-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Abstract The ability to accurately measure drug‐target interaction is critical for the discovery of new therapeutics. Classical pharmacological bioassays such as radioligand or fluorescent ligand binding assays can define the affinity or Kd of a ligand for a receptor with the lower the Kd, the stron...