Purification, Identification, and Properties of a Novel Carotenoid Produced by <i>Arthrobacter</i> sp. QL17 Isolated from Mount Qomolangma
The genus <i>Arthrobacter</i> is a source of many natural products that are critical in the development of new medicines. Here, we isolated a novel carotenoid from <i>Arthrobacter</i> sp. QL17 and characterized its properties. The carotenoid was extracted with methanol, and p...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Book |
| Published: |
MDPI AG,
2022-07-01T00:00:00Z.
|
| Subjects: | |
| Online Access: | Connect to this object online. |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
MARC
| LEADER | 00000 am a22000003u 4500 | ||
|---|---|---|---|
| 001 | doaj_7c6c6e4256c141c4b4533c3a5513f9fc | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Xue Yu |e author |
| 700 | 1 | 0 | |a Kan Jiang |e author |
| 700 | 1 | 0 | |a Wei Zhang |e author |
| 700 | 1 | 0 | |a Shuqing Dong |e author |
| 700 | 1 | 0 | |a Yujie Wu |e author |
| 700 | 1 | 0 | |a Gaosen Zhang |e author |
| 700 | 1 | 0 | |a Shiyu Wu |e author |
| 700 | 1 | 0 | |a Tuo Chen |e author |
| 700 | 1 | 0 | |a Guangxiu Liu |e author |
| 245 | 0 | 0 | |a Purification, Identification, and Properties of a Novel Carotenoid Produced by <i>Arthrobacter</i> sp. QL17 Isolated from Mount Qomolangma |
| 260 | |b MDPI AG, |c 2022-07-01T00:00:00Z. | ||
| 500 | |a 10.3390/antiox11081493 | ||
| 500 | |a 2076-3921 | ||
| 520 | |a The genus <i>Arthrobacter</i> is a source of many natural products that are critical in the development of new medicines. Here, we isolated a novel carotenoid from <i>Arthrobacter</i> sp. QL17 and characterized its properties. The carotenoid was extracted with methanol, and purified by column chromatography and semi-preparative HPLC. Based on micrOTOF-Q and NMR analyses, the pigment was chemically characterized as 2,2'-((((1E,3E,5E,7E,9E,11E,13E,15E,17E,19E)-3,7,14,18-tetramethylicosa-1,3,5,7,9,11,13,15,17,19-decaene-1,20-diyl)bis(2,2,4-trimethylcyclohex-3-ene-3,1-diyl)) bis(ethan-2-yl-1-ylidene))bi(propane-1,3-diol), and named arthroxanthin. The biological activities of arthroxanthin were evaluated with DPPH, ABTS and MTT assays. Arthroxanthin exhibited excellent radical scavenging properties, as shown for 2, 20-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-n-(3,2-ethyl-benzothiazole-6-sulfonic acid) ammonium salt (ABTS), respectively, with IC50s of 69.8 and 21.5 µg/mL. It also showed moderate anticancer activities against HepG2, Hela, MDAB-231, SW480, and MKN-45 with IC50 values of 107.6, 150.4, 143.4, 195.9, and 145.5 μg/mL, respectively. Therefore, arthroxanthin derived from <i>Arthrobacter</i> sp. QL17 may be a potent antioxidant and anticancer agent for food and pharmaceutical use. | ||
| 546 | |a EN | ||
| 690 | |a <i>Arthrobacter</i> | ||
| 690 | |a carotenoid | ||
| 690 | |a antioxidant | ||
| 690 | |a anticancer | ||
| 690 | |a Therapeutics. Pharmacology | ||
| 690 | |a RM1-950 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Antioxidants, Vol 11, Iss 8, p 1493 (2022) | |
| 787 | 0 | |n https://www.mdpi.com/2076-3921/11/8/1493 | |
| 787 | 0 | |n https://doaj.org/toc/2076-3921 | |
| 856 | 4 | 1 | |u https://doaj.org/article/7c6c6e4256c141c4b4533c3a5513f9fc |z Connect to this object online. |