Aktivitas antioksidan ekstrak daun tiga genus Artemisia sp dengan metode DPPH serta penetapan kadar total flavonoid, fenol dan karotenoid

• Main Authors: Wempi Budiana (Author), Aris Suhardiman (Author), Asep Roni (Author), Isnugraheni Sumarah (Author), Theodorik Erik Nara (Author)
• Format: Book
• Published: Universitas Jenderal Achmad Yani, 2017-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<p align="center"><strong>Abstrak</strong><strong></strong></p><p align="center"><strong> </strong></p><p>Tumbuhan adalah salah satu penghasil bahan berkhasiat obat salah satunya sebagai antioksidan. Senyawa pada tumbuhan yang bisa berfungsi sebagai antioksidan adalah flavonoid, fenol, dan karotenoid. Penelitian ini bertujuan untuk menetapkan aktivitas antioksidan dan  kadar flavonoid, fenol dan karotenoid total pada ekstrak daun  <em>Artemisia vulgaris </em>L.<em>, Artemisia annua  </em>L. dan <em> Artemisia dracunculus </em>L. Ekstraksi terhadap masing-masing bahan dilakukan secara bertingkat menggunakan alat refluks, pemantauan kandungan senyawa dengan Kromatografi Lapis Tipis (KLT), pengujian aktivitas antioksidan menggunakan DPPH, penetapan kadar flavonoid total menggunakan metode Ordon, penetapan kadar fenol total menggunakan reagen Folin-Ciocalteu dan penetapan kadar karotenoid total menggunakan pelarut <em>n</em>-heksana. Hasil menunjukkan ekstrak etanol daun<strong> </strong><em>Artemsia darcunculus</em> L. paling tinggi dalam menghambat radikal bebas dengan nilai IC₅₀ terkecil (32,10±0,10µg/mL), Ekstrak etanol daun<strong> </strong><em>Artemisia vulgaris</em> L. (77,19±0,13µg/mL) dan Ekstrak etanol daun<strong> </strong><em>Artemisia annua</em> L. (99,46±0,16 µg/mL).<em> </em>Kadar total flavonoid, fenolat dan karotenoid tertinggi pada ekstrak etil asetat daun <em>Artemisia vulgaris </em>L. berturut-turut 12,83 mg QE/100 mg ekstrak, 68,72 mg GAE/100 mg ekstrak dan 10,01 mg BE/100 mg ekstrak. Dapat disimpulkan bahwa<strong> e</strong>kstrak etanol daun<strong> </strong><em>Artemisia </em><em>darcunculus</em><em> </em>L. memiliki aktivitas antioksidan paling kuat dan ekstrak etanol daun<strong> </strong><em>Artemisia vulgaris </em>L. memiliki kadar flavonoid total, fenolat total dan karotenoid total tertinggi.</p><p> </p><p><strong>Kata Kunci:</strong> <em>Artemisia sp</em>, antioksidan, flavonoid, fenol, karotenoid.</p><p> </p><p align="center"><strong><em>Antioxidant Activities of Leaves Extract Three Genus Artemisia sp Using DPPH Method and Total Content of Flavonoid, Fenol And Carotenoid</em></strong></p><p> </p><p align="center"><strong><em>Abstract</em></strong><strong><em></em></strong></p><p align="center"><strong><em> </em></strong></p><p><em>P</em><em>lant is </em><em>the natural product medicine such as</em><em> antioxidant. </em><em>Natural compounds </em><em>as antioxidants are flavonoids, phenols, and carotenoids.</em><em> </em><em>T</em><em>his research was conducted to determine</em><em>the antioxidant activities and</em><em> the levels of</em><em> total flavonoids, phenols and carotenoids</em><em> content</em><em> in</em><em> </em><em>Artemisia vulgaris L., Artemisia annua L. and Artemisia dracunculus L.</em><em> extract.</em><em> </em><em>Each sample was extracted gradual </em><em>reflux</em><em> method</em><em>, monitoring</em><em> of extracts compounds with</em><em> Thin Layer Chromatography (TLC), antioxidant activities were test using DPPH, </em><em>determination</em><em> of total flavonoids</em><em> content with </em><em>Ordon method, total phenol content</em><em> with </em><em>Folin-Ciocalteu reagent and</em><em> </em><em>total carotenoids</em><em> using n</em><em>-hexane. </em><em>Ethanol extract of Leaves Artemisia dracunculus L. was highest in inhibiting free radicals with the smallest IC₅₀ value (32.10±0.10μg / mL), ethanol extract of leaves Artemisia vulgaris L. (77.19 ± 0.13μg / mL) and ethanol extract of leaves Artemisia annua L. (99.46±0.16 μg / mL). The highest total content of flavonoid, phenolate and carotenoid in ethyl acetate extract of leaves Artemisia vulgaris L. were 12.83 mg QE / 100 mg extract, 68.72 mg GAE / 100 mg extract and 10.01 mg BE / 100 mg extract. Ethanol extract of Leaves Artemisia darcunculus L. has the strongest antioxidant activity, ethanolic extract of leaves Artemisia vulgaris L. has highest total flavonoid content, total phenolate and total carotenoid.</em><em></em></p><p><em> </em></p><p><strong><em>Keyword</em></strong><strong><em>s</em></strong><strong><em>:</em></strong><strong><em>              </em></strong><em>Artemisia </em><em>sp</em><em>,</em><em> antioxidant, </em><em>flavonoid, phenol</em><em>ic</em><em>, carotenoid </em><strong><em></em></strong></p>

Item Description

2354-6565
2502-3438
10.26874/kjif.v5i2.106