ESTIMATION OF METHIONINE AND LYSINE IN PROTEIN BY THIN-LAYER CHROMATOGRAPHY
A thin-layer chromatography method has been developed and evaluated for the separation and determination of lysine and methonine in a mixture of amino acids obtained from acid or enzyme hydrolysis of protein. The solvent systems were (a) ethanol-ether, (b) phenol-water, (c) ethyl methyl ketone-pyrid...
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| Main Authors: | , |
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| Format: | Book |
| Published: |
Tehran University of Medical Sciences,
1983-08-01T00:00:00Z.
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| Summary: | A thin-layer chromatography method has been developed and evaluated for the separation and determination of lysine and methonine in a mixture of amino acids obtained from acid or enzyme hydrolysis of protein. The solvent systems were (a) ethanol-ether, (b) phenol-water, (c) ethyl methyl ketone-pyridine-distilled water-glacial acetic acid. Procedures in all the three stages were in the same dimension. Results of casein analysis by this method showed ± 15% fluctuations when compared with standard amino acid analyzer. |
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| Item Description: | 2251-6085 |