Characterization and Anticancer Activity from Gaharu (Aquilaria malaccensis) Stem Bark Extract
<p><em>Aquilaria malaccensis</em> is a plant species from the Thymeleaceae family. <em>A. malaccensis</em> contains secondary metabolites of phenolic groups, namely benzophenone, chromone, coumarin, xanthone, and flavonoids with various biological activities such as ant...
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Universitas Sultan Ageng Tirtayasa,
2021-12-01T00:00:00Z.
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| Summary: | <p><em>Aquilaria malaccensis</em> is a plant species from the Thymeleaceae family. <em>A. malaccensis</em> contains secondary metabolites of phenolic groups, namely benzophenone, chromone, coumarin, xanthone, and flavonoids with various biological activities such as antioxidants and anticancer. This study aims to determine the potential of <em>A. malaccensis</em> stem bark extract as an anticancer and to determine the characteristics of secondary metabolite compounds contained in <em>A. malaccensis</em> stem bark extract. Extraction of the stem bark of <em>A. malaccensis</em> by maceration method in stages using <em>n</em>-hexane, ethyl acetate and methanol as solvents. The anticancer activity test used the microculture tetrazolium (MTT) assay against Murine Leukemia P-388 cells. Compound separation using thin layer chromatograph (TLC) and gravity column chromatography (CC) methods. Determination of chemical compounds using liquid chromatography mass spectroscopy/mass spectroscopy (LCMS/MS). The extracts of <em>n</em>-hexane, ethyl acetate, and methanol each had anticancer activity which was classified as active with an IC<sub>50</sub> value of 20.64, 26.61, and 14.49 µg/mL. Based on the results of separation and identification of compounds in methanol extract with TLC, CC, and LCMS/MS, methanol extract contains acetaminophen, aminosamptothesin, isophorone, diethyl phthalate, 3-hydroxy-6H-benzochromen-6-one, isolongifolenic, 2-(2-phenethyl) chromone, 4',7-dihydroxy-2',5-dimethoxyisoflavone, 4-hydroxy-3-(1-phenylpropyl)-2H-chromone-2-one; 6-methylchromone-2-carboxylic acid; 3,4-dimethyl-2- phenylmorpholine; and β-calacoren compounds.</p> |
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| Item Description: | 2502-4779 2502-4787 10.30870/educhemia.v6i2.10983 |