Evaluation of semi-automated cells counting in peritoneal fluid
ABSTRACTIntroduction:Currently, the cytological analysis of biological fluids, such as peritoneal fluid, is performed by manually cells counting in Fuchs-Rosenthal chamber. However, this method has a number of limitations. Because of these limitations, automatic counters have been evaluated for cell...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Book |
| Published: |
Sociedade Brasileira de Patologia Clínica,
2015-08-01T00:00:00Z.
|
| Subjects: | |
| Online Access: | Connect to this object online. |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | ABSTRACTIntroduction:Currently, the cytological analysis of biological fluids, such as peritoneal fluid, is performed by manually cells counting in Fuchs-Rosenthal chamber. However, this method has a number of limitations. Because of these limitations, automatic counters have been evaluated for cell counting in this type of sample in order to make it faster and more reliable test.Objective:The aim of this study is to compare the manual and semi-automated leukocytes and erythrocytes counting in peritoneal fluid.Materials and methods:The samples were analyzed manually and using the CountessTM(Invitrogen).Results:The results showed that although there is a correlation between the two counting methods, the correlation is relatively low, for both leukocytes and erythrocytes analysis.Conclusion:The results suggest that peritoneal fluid should continue to be analyzed in Fuchs-Rosenthal chamber. However, further studies should be conducted with a greater number of samples to investigate the possibility of using automated cells counting in serous fluids and, thus, provide greater speed and quality of results. |
|---|---|
| Item Description: | 1678-4774 10.5935/1676-2444.20150037 |