Speciation and Antibiotic Susceptibilities of Coagulase Negative Staphylococci Isolated from Ocular Infections

• Main Authors: John E. Romanowski (Author), Shannon V. Nayyar (Author), Eric G. Romanowski (Author), Vishal Jhanji (Author), Robert M. Q. Shanks (Author), Regis P. Kowalski (Author)
• Format: Book
• Published: MDPI AG, 2021-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.3390/antibiotics10060721; 2079-6382
• Keywords: coagulase-negative staphylococci; eye infections; endophthalmitis; keratitis; conjunctivitis; blepharitis; Therapeutics. Pharmacology; RM1-950
• Source: Antibiotics, Vol 10, Iss 6, p 721 (2021)

Coagulase-negative staphylococci (CoNS) are frequently occurring ocular opportunistic pathogens that are not easily identifiable to the species level. The goal of this study was to speciate CoNS and document antibiotic susceptibilities from cases of endophthalmitis (<i>n</i> = 50), keratitis (<i>n</i> = 50), and conjunctivitis/blepharitis (<i>n</i> = 50) for empiric therapy. All 150 isolates of CoNS were speciated using (1) API Staph (biochemical system), (2) Biolog GEN III Microplates (phenotypic substrate system), and (3) DNA sequencing of the <i>sodA</i> gene. Disk diffusion antibiotic susceptibilities for topical and intravitreal treatment were determined based on serum standards. CoNS identification to the species level by all three methods indicated that <i>S. epidermidis</i> was the predominant species of CoNS isolated from cases of endophthalmitis (84-90%), keratitis (80-86%), and conjunctivitis/blepharitis (62-68%). Identifications indicated different distributions of CoNS species among endophthalmitis (6), keratitis (10), and conjunctivitis/blepharitis (13). Antibiotic susceptibility profiles support empiric treatment of endophthalmitis with vancomycin, and keratitis treatment with cefazolin or vancomycin. There was no clear antibiotic choice for conjunctivitis/blepharitis. <i>S. epidermidis</i> was the most frequently found CoNS ocular pathogen, and infection by other CoNS appears to be less specific and random. Antibiotic resistance does not appear to be a serious problem associated with CoNS.