Micronucleus frequency in exfoliated buccal cells from hairdresser who expose to hair products
<p><strong><em></em></strong><em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Background:</strong><span style="font-family: Tribune-Italic; font-size...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Book |
| Published: |
Universitas Airlangga,
2015-06-01T00:00:00Z.
|
| Subjects: | |
| Online Access: | Connect to this object online. |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | <p><strong><em></em></strong><em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Background:</strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em> Hairdresser is one of the fastest growing occupations in today's society. Hairdresser help styling, cutting, colouring, </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>perming, curling, straightening hair and various treatment to customer. Somehow, hairdresser are constantly exposed to chemical </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>substances such as aromatic amines, hydrogen peroxide, thioglycolic acid, formaldehyde in hair products which can cause damage </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>to human's genome. Micronucleus is one of the effective biomarker for processes associated with the induction of DNA damage. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Purpose: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The aim of this study was to determine the micronucleus frequencies in buccal mucosa epithelial cells of hairdresser who </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>were exposed to chemical of hair products. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Method:</strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em> This study was conducted on twenty female subjects, who were divided into 2 </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>groups: exposed and non-exposed (control) group. All subjects recruited were working in the same beauty salon. Buccal cells were </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>obtained from each individual by using cytobrush. The cells were stained with modified Feulgen-Ronssenback method and counting </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>of micronucleus per 1000 cell was done under light microscope. The data were analyzed using independent t-test and one-way Anova </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>(p<0.05). </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Result: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The result showed a significant difference in micronucleus frequency between 2 groups. There were a significantly </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>increase of micronucleus frequency in hairdressers and increase of micronucleus frequency with the longer duration of exposure. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Conclusion:</strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em> It concluded that the chemical substances of hair products had affected the micronucleus frequency ofthe epithelial cells </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>in buccal mucosa of hairdressers.</em></span></span></span></span></span></span></span></span></span></span></span></span><br style="font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" /></span></span></span></span></span></span></span></span></em></p> |
|---|---|
| Item Description: | 1978-3728 2442-9740 10.20473/j.djmkg.v48.i2.p74-79 |