The study of phospholipid bilayer of cell membranes in leukocytes incubated with high concentrations of the food additive E407a
<b>The aim </b>of the study was to assess the impact of semi-refined carrageenan (E407a) on the hydrophobic region of phospholipid bilayer in cell membranes of leukocytes exposed to the solution with high concentrations of this food additive.<br /> <b>Materials and methods.&l...
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| Main Authors: | , , , |
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| Format: | Book |
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National Scientific Medical Center,
2021-04-01T00:00:00Z.
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| Summary: | <b>The aim </b>of the study was to assess the impact of semi-refined carrageenan (E407a) on the hydrophobic region of phospholipid bilayer in cell membranes of leukocytes exposed to the solution with high concentrations of this food additive.<br /> <b>Materials and methods.</b> Fluorescent probe (2-phenyl-phenanthro[9,10-<i>d</i>]-1,3-oxazole) was used to investigate the influence of E407a on the state of lipid bilayer in leukocytes extracted from rats and treated with a 5% solution of the food additive E407a during 4 hours. <br /> <b>Results.</b> The shapes of the probe fluorescence spectra did not differ in leukocytes of rats treated with the 5% solution of semi-refined carrageenan and white blood cells of control samples. Such findings suggest that exposure to the E407a solution causes no changes in the <i>proton-donor ability</i> of the media in the lipid membranes of leukocytes in the region where the probe locates.<br /> <b>Conclusion.</b> Exposure of white blood cell suspensions to the semi-refined carrageenan solution does not affect the membrane hydration of the hydrophobic region of leukocyte phospholipid bilayer.<br /> |
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| Item Description: | 1812-2892 2313-1519 10.23950/jcmk/10799 |