Dynamic Gene Clusters Mediating Carbapenem-Resistant <i>Acinetobacter baumannii</i> Clinical Isolates
<i>Acinetobacter baumanni</i> (<i>A. baumannii</i>), a nonfermenting Gram-negative bacterium, has recently been associated with a broad range of nosocomial infections. To gain more meaningful insight into the problem of nosocomial illnesses caused by the multidrug-resistant (...
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| Main Authors: | , , , , , |
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| Format: | Book |
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MDPI AG,
2022-01-01T00:00:00Z.
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| Summary: | <i>Acinetobacter baumanni</i> (<i>A. baumannii</i>), a nonfermenting Gram-negative bacterium, has recently been associated with a broad range of nosocomial infections. To gain more meaningful insight into the problem of nosocomial illnesses caused by the multidrug-resistant (MDR) <i>A. baumannii</i>, as well as the factors that increase the risk of catching these infections, this investigation included a total of 86 clinical <i>A. baumannii</i> infections. Repetitive extragenic palindromic (REP)-PCR was used to investigate imipenem-resistant <i>A. baumannii</i> isolates for dynamic gene clusters causing carbapenem resistance. Four distinct <i>A. baumannii</i> lineages were found in the REP-PCR-DNA fingerprints of all isolates, with 95% of the samples coming from two dominant lineages. Imipenem, amikacin, and ciprofloxacin were less effective against genotype (A) isolates because of enhanced antibiotic tolerance. Lastly, to gain more insight into the mode of action of imipenem, we explored the binding affinity of imipenem toward different <i>Acinetobacter baumannii</i> OXA beta-lactamase class enzymes. |
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| Item Description: | 10.3390/antibiotics11020168 2079-6382 |