The Anti-Microbial Peptide (Lin-SB056-1)₂-K Reduces Pro-Inflammatory Cytokine Release through Interaction with <i>Pseudomonas aeruginosa</i> Lipopolysaccharide

• Main Authors: Lucia Grassi (Author), Arianna Pompilio (Author), Esingül Kaya (Author), Andrea C. Rinaldi (Author), Enrico Sanjust (Author), Giuseppantonio Maisetta (Author), Aurélie Crabbé (Author), Giovanni Di Bonaventura (Author), Giovanna Batoni (Author), Semih Esin (Author)
• Format: Book
• Published: MDPI AG, 2020-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

The ability of many anti-microbial peptides (AMPs) to modulate the host immune response has highlighted their possible therapeutic use to reduce uncontrolled inflammation during chronic infections. In the present study, we examined the anti-inflammatory potential of the semi-synthetic peptide lin-SB056-1 and its dendrimeric derivative (lin-SB056-1)₂-K, which were previously found to have anti-microbial activity against <i>Pseudomonas aeruginosa</i> in in vivo-like models mimicking the challenging environment of chronically infected lungs (i.e., artificial sputum medium and 3-D lung mucosa model). The dendrimeric derivative exerted a stronger anti-inflammatory activity than its monomeric counterpart towards lung epithelial- and macrophage-cell lines stimulated with <i>P. aeruginosa</i> lipopolysaccharide (LPS), based on a marked decrease (up to 80%) in the LPS-induced production of different pro-inflammatory cytokines (i.e., IL-1β, IL-6 and IL-8). Accordingly, (lin-SB056-1)₂-K exhibited a stronger LPS-binding affinity than its monomeric counterpart, thereby suggesting a role of peptide/LPS neutralizing interactions in the observed anti-inflammatory effect. Along with the anti-bacterial and anti-biofilm properties, the anti-inflammatory activity of (lin-SB056-1)₂-K broadens its therapeutic potential in the context of chronic (biofilm-associated) infections.

Item Description

10.3390/antibiotics9090585
2079-6382