The Intestinal Carriage of Plasmid-Mediated Colistin-Resistant Enterobacteriaceae in Tertiary Care Settings
<b>Background:</b> In order to estimate the prevalence of plasmid borne colistin resistance and to characterize in detail the <i>mcr</i>-positive isolates, we carried out a sentinel testing survey on the intestinal carriage of plasmid-mediated colistin-resistant Enterobacteri...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Book |
| Published: |
MDPI AG,
2021-03-01T00:00:00Z.
|
| Subjects: | |
| Online Access: | Connect to this object online. |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | <b>Background:</b> In order to estimate the prevalence of plasmid borne colistin resistance and to characterize in detail the <i>mcr</i>-positive isolates, we carried out a sentinel testing survey on the intestinal carriage of plasmid-mediated colistin-resistant Enterobacteriaceae in hospitalized patients. <b>Methods:</b> Between June 2018 and September 2019, 1922 faecal samples from hospitalised patients were analysed by selective culture in presence of colistin (3.5 mg/L), and in parallel by direct detection of the <i>mcr-1</i> to <i>mcr-8</i> genes by qPCR. The <i>mcr</i>-positive isolates were characterised by whole-genome sequencing. <b>Results:</b> The prevalence of the <i>mcr-1</i> gene was 0.21% (<i>n</i> = 4/1922); the <i>mcr-2</i> to <i>8</i> genes were not detected. The <i>mcr-1</i> gene was found to be localised in the IncX4 (<i>n</i> = 3) and IncHI2 (<i>n</i> = 1) plasmid type. One <i>Escherichia coli</i> isolate was susceptible to colistin due to the inactivation of the <i>mcr-1</i> gene through the insertion of the IS2 element; however, the colistin resistance was inducible by culture in low concentrations of colistin. One human <i>mcr-1</i> positive <i>E. coli</i> isolate was related genetically to the <i>mcr-1 E. coli</i> isolate derived from turkey meat of Czech origin. <b>Conclusions:</b><i>mcr</i>-mediated colistin resistance currently poses little threat to patients hospitalised in Czech healthcare settings. The presence of the <i>mcr-1</i> gene in the human population has a possible link to domestically produced, retail meat. |
|---|---|
| Item Description: | 10.3390/antibiotics10030258 2079-6382 |