Study on apoptosis effect of human breast cancer cell MCF-7 induced by lycorine hydrochloride via death receptor pathway

• Main Authors: Yubin Ji (Author), Miao Yu (Author), Zheng Qi (Author), Di Cui (Author), Guosong Xin (Author), Bing Wang (Author), Weiling Jia (Author), Lin Chang (Author)
• Format: Book
• Published: Elsevier, 2017-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

As research was conducted on the early apoptosis of human breast cancer cell MCF-7 caused by lycorine hydrochloride and the expression of the related apoptosis proteins. The early-period apoptosis rate of human breast cancer cell MCF-7 was tested with the AnnexinV/PI double staining and flow cytometry. The Western Blotting method was also used to detect the protein expression conditions of Fas, FasL, Caspase-8 and Bid. The results showed that the higher the dose, the higher the rate of apoptosis and that the rate of apoptosis was dependent on the dose; the relative protein activity of Fas, FasL, Caspase-8 and bid gradually rose with the increase of lycorine dosage and the activities revealed certain dose-independence. Results showed that lycorine hydrochloride could induce the apoptosis of human breast cancer cell MCF-7 through the death receptor pathway. Keywords: Lycorine hydrochloride, MCF-7, Death receptor pathway, Apoptosis

Item Description

1319-0164
10.1016/j.jsps.2017.04.036