Prion protein and its interactions with metal ions (Cu2+, Zn2+, and Cd2+) and metallothionein 3

• Main Authors: Branislav Ruttkay-Nedecky (Author), Eliska Sedlackova (Author), Dagmar Chudobova (Author), Kristyna Cihalova (Author), Ana Maria Jimenez Jimenez (Author), Sona Krizkova (Author), Lukas Richtera (Author), Vojtech Adam (Author), Rene Kizek (Author)
• Format: Book
• Published: International Association of Physical Chemists (IAPC), 2015-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The effects of heavy metals (Zn2+, Cu2+, and/or Cd2+) on Escherichia coli expressing either prion (hPrPC ) or metallothionein 3 (MT-3) brain proteins capable of binding these metals were investigated. The expression of hPrPC or MT-3 in E.coli was confirmed using western-blot and dot-blot methods. Af...