Lipopolysaccharide increases lysozyme adhesion to chitin
<p><span style="text-decoration: underline;">Background.</span> To study the effect of lipopolysaccharide on the adsorption of lysozyme on chitin.</p><p><span style="text-decoration: underline;">Methods.</span> Egg white lysozyme, lipopol...
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| Main Authors: | , , , , |
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| Format: | Book |
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Kazimierz Wielki University,
2019-11-01T00:00:00Z.
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| Summary: | <p><span style="text-decoration: underline;">Background.</span> To study the effect of lipopolysaccharide on the adsorption of lysozyme on chitin.</p><p><span style="text-decoration: underline;">Methods.</span> Egg white lysozyme, lipopolysaccharide and chitin were used. The lyophilized cells of <em>M. lyzodeikticus</em> were used as a lysozyme substrate. Lysozyme activity was determined by the bacteriolytic method using different concentrations (8-512 μg/ml). Lysozyme adsorption on chitin was determined by the loss of lysozyme from solution after shaking with chitin. The effect of lipopolysaccharide (0.6-40 μg/ml) on lysozyme adsorption was evaluated by the loss of lysozyme from the solution after shaking with 50 mg of chitin.</p><p><span style="text-decoration: underline;">Results.</span> In determining the activity of lysozyme by the bacteriolytic method, the linear nature of the dependence of activity on the concentration of the enzyme is observed only at low concentrations (8-100 μg / ml). Lipopolysaccharide does not affect the bacteriolytic activity of lysozyme, however, dose-dependently increases the adsorption of lysozyme on chitin.</p><p><span style="text-decoration: underline;">Conclusion.</span> Lipopolysaccharide increases the adsorption of lysozyme on chitin.</p> |
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| Item Description: | 2391-8306 10.12775/JEHS.2019.09.11.021 |