Oropouche virus cases identified in Ecuador using an optimised qRT-PCR informed by metagenomic sequencing.

• Main Authors: Emma L Wise (Author), Sully Márquez (Author), Jack Mellors (Author), Verónica Paz (Author), Barry Atkinson (Author), Bernardo Gutierrez (Author), Sonia Zapata (Author), Josefina Coloma (Author), Oliver G Pybus (Author), Simon K Jackson (Author), Gabriel Trueba (Author), Gyorgy Fejer (Author), Christopher H Logue (Author), Steven T Pullan (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2020-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Oropouche virus (OROV) is responsible for outbreaks of Oropouche fever in parts of South America. We recently identified and isolated OROV from a febrile Ecuadorian patient, however, a previously published qRT-PCR assay did not detect OROV in the patient sample. A primer mismatch to the Ecuadorian O...