Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway

• Main Authors: YI Jianru (Author), LI MeiLe (Author), Yan YANG (Author), Wei ZHENG (Author), LI Yu (Author), Zhihe ZHAO (Author)
• Format: Book
• Published: University of São Paulo, 2015-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 1678-7765; 10.1590/1678-775720150156
• Keywords: Osteogenesis; Periodontal ligament; Calcium-calmodulin-dependent Protein kinases; Mechanotransduction; Dentistry; RK1-715
• Source: Journal of Applied Oral Science, Vol 23, Iss 6, Pp 549-554 (2015)

ABSTRACT Objective This study aimed to investigate the potential role of CAMK II pathway in the compression-regulated OPG expression in periodontal ligament cells (PDLCs). Material and Methods The PDL tissue model was developed by 3-D culturing human PDLCs in a thin sheet of poly lactic-co-glycolic acid (PLGA) scaffolds, which was subjected to static compression of 25 g/cm2 for 3, 6 and 12 h, with or without treatment of KN-93. After that, the expression of OPG, RANKL and NFATC2 was investigated through real-time PCR and western blot analysis. Results After static compression, the NFATC2 and RANKL expression was significantly up-regulated, while partially suppressed by KN-93 for 6 and 12 h respectively. The OPG expression was significantly down-regulated by compression in 3 h, started to elevate in 6 h, and significantly up-regulated in 12 h. The up-regulation after 12 h was significantly suppressed by KN-93. Conclusions Long-term static compression increases OPG expression in PDLCs, at least partially, via the CAMK II pathway.