Differential regulation of C-C chemokines during fibroblast-monocyte interactions: adhesion vs. inflammatory cytokine pathways
The cell-to-ce ll interactions during chronic inflammatory diseases likely contribute to leukocyte accumulation leading to increased pathology and organ dys -function. In particular, there is a paucity of information relating to the maintenance of chronic fibrotic diseases . Using a lung fibroblast...
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| Main Authors: | , , , , , , |
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| Format: | Book |
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Hindawi Limited,
1998-01-01T00:00:00Z.
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| Summary: | The cell-to-ce ll interactions during chronic inflammatory diseases likely contribute to leukocyte accumulation leading to increased pathology and organ dys -function. In particular, there is a paucity of information relating to the maintenance of chronic fibrotic diseases . Using a lung fibroblast line and enriched monocyte populations , we have investigated the activational events which contribute to the production of two C-C chemokines , macrophage inflammatory protein-1 alpha (MIP-1α ) and monocyte chemoattractant protein-1 (MCP-1), during fibroblas tmonocyte interactions . Neither the fibroblast cell line (16 lu) nor isolated monocytes alone produced significant levels of MIP-1α or MCP-1. However, when isolated monocytes were layered onto 16 lu fibroblas tmonolayers as ignificant increase in MIP-1α and MCP- 1 production was observed. The use of fixed cell populations indicated that the MIP-1α was derived from monocytes and MCP-1 from both cell populations. To examine the molecules which wer erequired for chemokine production during the interaction, specific antibodies were used in the co-cultures . Blocking β3-integrin interactions s ignificantly inhibited MIP-1α production. In contrast, beta-integr in interactions had no effect on the MCP-1 production, while, neutralization of TNF significantly decreased MCP-1 production during the co-culture . These data indicate that fibroblast-monocyte inte ractions induce chemokine production through different mechanisms and a combination of these responses may contribute to the maintenance of the mononuclear cell accumulation during diseaseprogression. |
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| Item Description: | 0962-9351 1466-1861 10.1080/09629359890956 |