HPLC-DAD assay of phenols profile in Antennaria dioica (L.) Gaertn
Antennaria dioica (Asteraceae family) - is a perennial herb, commonly found in dry grasslands and sandy or stony places from Eurasian areas. It is known in traditional medicine as antioxidant, diuretic, choleretic and anti-inflammatory remedy. This species should be reconsidered as possible sources...
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| Main Authors: | , , , , |
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| Format: | Book |
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Pensoft Publishers,
2022-05-01T00:00:00Z.
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| Summary: | Antennaria dioica (Asteraceae family) - is a perennial herb, commonly found in dry grasslands and sandy or stony places from Eurasian areas. It is known in traditional medicine as antioxidant, diuretic, choleretic and anti-inflammatory remedy. This species should be reconsidered as possible sources of phenols, mainly flavonoids and hydroxycinnamic acids. Thus, the aim of this study was to validate a chromatographic method for detection of phenols and their identification in A. dioica herb. HPLC-DAD method was evaluated in terms of linearity, precision, repeatability, accuracy, LOD and LOQ. The calibration curves of thirteen reference substances were linear (R2 > 0.99) over the range of 5-400 µg/mL, the LODs and the LOQs were in the range of 0.1-0.3 µg/mL and 0.2-1.0 µg/mL, respectively. During HPLC-DAD assay two flavones - luteolin, apigenin; flavonol - quercetin and three its glycosides - rutin, hyperoside and isoquercitrin; coumarins: coumarin and umbelliferone; five hydroxycinnamic acids - chlorogenic, caffeic, p-coumaric, trans-ferulic and rosmarinic were identified in A. dioica herb. This phytochemical study of A. dioica confirms that this plant material is a rich source of phenolic compounds. |
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| Item Description: | 10.3897/pharmacia.69.e81654 2603-557X |