Antibacterial activity of silver nanoparticles immobilized in zinc oxide-eugenol cement against Enterococcus Faecalis: an in vitro study

• Main Authors: Juan Camilo Duque-Aristizábal (Author), Liza María Isaza-Areiza (Author), Diego Tobón-Calle (Author), Martha Elena Londoño-López (Author)
• Format: Book
• Published: Universidad de Antioquia, 2019-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: https://doi.org/10.17533/udea.rfo.v30n2a2; 0121-246X; 2145-7670
• Keywords: endodontics; sealing; cements; microorganisms; enterococcus faecalis; kirby-bauer; nanotechnology; in-vitro; endodoncia; obturación; cementos; microorganismos; enterococcus faecalis; kirby-bauer; nanotecnología; Dentistry; RK1-715
• Source: Revista Facultad de Odontología Universidad de Antioquia, Vol 30, Iss 2, Pp 154-165 (2019)

Introduction: the aim of this study is to evaluate the antimicrobial capacity of silver nanoparticles immobilized in a zinc oxide-eugenol (ZOE) cement against Enterococcus faecalis for potential use in endodontic treatments. Method: experimental in vitro study, performing synthesis of silver nanoparticles (AgNPs) and UV-visible spectroscopy to confirm presence of AgNPs in the prepared solutions. The ZOE mixture was standardized, producing the integrated AgNPs/ZOE material. Scanning electron microscopy (SEM) and Fourier-Transform Infrared Spectroscopy (FTIR) were used to characterize the integrated material; a KirbyBauer assay was finally run to measure the inhibition halos produced by the compound-microorganism interaction. Results: the UV-visible spectroscopy showed presence of AgNPs in the created solution; both SEM and FTIR show that the AgNPs are integrated into the ZOE system, not altering their properties when performed under conditions like those found in the mouth. The Kirby-Bauer assay shows that all samples had inhibition halos. The AgNPs in guava extract had statistically significant differences with the halos of the other samples (p < 0.05). Conclusions: the obtained AgNPs show bactericidal activity against Enterococcus faecalis, as a statistically significant difference was found between the AgNPs suspended in guava extract and the other groups; this will be the starting point for future research