Nanovectorized Microalgal Extracts to Fight <i>Candida albicans</i> and <i>Cutibacterium acnes</i> Biofilms: Impact of Dual-Species Conditions
Biofilm-related infections are a matter of concern especially because of the poor susceptibility of microorganisms to conventional antimicrobial agents. Innovative approaches are needed. The antibiofilm activity of extracts of cyanobacteria <i>Arthrospira platensis</i>, rich in free fatt...
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| Main Authors: | , , , , , , , |
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| Format: | Book |
| Published: |
MDPI AG,
2020-05-01T00:00:00Z.
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| Online Access: | Connect to this object online. |
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| Summary: | Biofilm-related infections are a matter of concern especially because of the poor susceptibility of microorganisms to conventional antimicrobial agents. Innovative approaches are needed. The antibiofilm activity of extracts of cyanobacteria <i>Arthrospira platensis</i>, rich in free fatty acids, as well as of extract-loaded copper alginate-based nanocarriers, were studied on single- and dual-species biofilms of <i>Candida albicans</i> and <i>Cutibacterium acnes</i>. Their ability to inhibit the biofilm formation and to eradicate 24 h old biofilms was investigated. Concentrations of each species were evaluated using flow cytometry. Extracts prevented the growth of <i>C. acnes</i> single-species biofilms (inhibition > 75% at 0.2 mg/mL) but failed to inhibit preformed biofilms. Nanovectorised extracts reduced the growth of single-species <i>C. albicans</i> biofilms (inhibition > 43% at 0.2 mg/mL) while free extracts were weakly or not active. Nanovectorised extracts also inhibited preformed <i>C. albicans</i> biofilms by 55% to 77%, whereas the corresponding free extracts were not active. In conclusion, even if the studied nanocarrier systems displayed promising activity, especially against <i>C. albicans</i>, their efficacy against dual-species biofilms was limited. This study highlighted that working in such polymicrobial conditions can give a more objective view of the relevance of antibiofilm strategies by taking into account interspecies interactions that can offer additional protection to microbes. |
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| Item Description: | 10.3390/antibiotics9060279 2079-6382 |