Proteomics-Based RT-qPCR and Functional Analysis of 18 Genes in Metronidazole Resistance of <i>Bacteroides fragilis</i>
Previously, we reported that metronidazole MICs are not dependent on the expression levels of <i>nim</i> genes in <i>B. fragilis</i> strains and we compared the proteomes of metronidazole-resistant laboratory <i>B. fragilis</i> strains to those of their susceptibl...
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2024-02-01T00:00:00Z.
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LEADER | 00000 am a22000003u 4500 | ||
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001 | doaj_8a72e60a3cd042b09b69d13b8e800ca5 | ||
042 | |a dc | ||
100 | 1 | 0 | |a Bakhtiyar Mahmood |e author |
700 | 1 | 0 | |a Ana Paunkov |e author |
700 | 1 | 0 | |a Malgorzata Kupc |e author |
700 | 1 | 0 | |a Katalin Burián |e author |
700 | 1 | 0 | |a Elisabeth Nagy |e author |
700 | 1 | 0 | |a David Leitsch |e author |
700 | 1 | 0 | |a József Sóki |e author |
245 | 0 | 0 | |a Proteomics-Based RT-qPCR and Functional Analysis of 18 Genes in Metronidazole Resistance of <i>Bacteroides fragilis</i> |
260 | |b MDPI AG, |c 2024-02-01T00:00:00Z. | ||
500 | |a 10.3390/antibiotics13030207 | ||
500 | |a 2079-6382 | ||
520 | |a Previously, we reported that metronidazole MICs are not dependent on the expression levels of <i>nim</i> genes in <i>B. fragilis</i> strains and we compared the proteomes of metronidazole-resistant laboratory <i>B. fragilis</i> strains to those of their susceptible parent strains. Here, we used RT-qPCR to correlate the expression levels of 18 candidate genes in a panel of selected, clinical <i>nim</i> gene-positive and -negative <i>B. fragilis</i> strains to their metronidazole MICs. Metronidazole MICs were correlated with the expression of certain tested genes. Specifically, lactate dehydrogenase expression correlated positively, whereas cytochrome fumarate reductase/succinate dehydrogenase, malate dehydrogenase, phosphoglycerate kinase redox and <i>gat</i> (GCN5-like acetyltransferase), and <i>relA</i> (stringent response) regulatory gene expressions correlated negatively with metronidazole MICs. This result provides evidence for the involvement of carbohydrate catabolic enzymes in metronidazole resistance in <i>B. fragilis</i>. This result was supported by direct substrate utilization tests. However, the exact roles of these genes/proteins should be determined in deletion-complementation tests. Moreover, the exact redox cofactor(s) participating in metronidazole activation need to be identified. | ||
546 | |a EN | ||
690 | |a <i>Bacteroides</i> | ||
690 | |a metronidazole | ||
690 | |a <i>nim</i> | ||
690 | |a resistance mechanism | ||
690 | |a RT-qPCR | ||
690 | |a Therapeutics. Pharmacology | ||
690 | |a RM1-950 | ||
655 | 7 | |a article |2 local | |
786 | 0 | |n Antibiotics, Vol 13, Iss 3, p 207 (2024) | |
787 | 0 | |n https://www.mdpi.com/2079-6382/13/3/207 | |
787 | 0 | |n https://doaj.org/toc/2079-6382 | |
856 | 4 | 1 | |u https://doaj.org/article/8a72e60a3cd042b09b69d13b8e800ca5 |z Connect to this object online. |