Lipid-rich fraction of the sclerotium of Tiger Milk Mushroom Lignosus rhinocerotis (Agaricomycetes) attenuates LPS-induced inflammation in BV2 cells via Nrf2 pathway
Lignosus rhinocerotis (tiger milk mushroom) is widely used by the indigenous people of Malaysia as a traditional remedy. The present study was carried out in order to evaluate the antioxidant, cytotoxic and anti-neuroinflammatory activities of L. rhinocerotis extract on brain microglial cells (BV2)....
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Universidade de São Paulo,
2020-06-01T00:00:00Z.
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LEADER | 00000 am a22000003u 4500 | ||
---|---|---|---|
001 | doaj_8a72f7064e4b454886d01ac865da9cf2 | ||
042 | |a dc | ||
100 | 1 | 0 | |a Neeranjini Nallathamby |e author |
700 | 1 | 0 | |a Hariprasath Lakshmanan |e author |
700 | 1 | 0 | |a Sharmili Vidyadaran |e author |
700 | 1 | 0 | |a Sri Nurestri Abdul Malek |e author |
700 | 1 | 0 | |a Chia-Wei Phan |e author |
700 | 1 | 0 | |a Vikineswary Sabaratnam |e author |
245 | 0 | 0 | |a Lipid-rich fraction of the sclerotium of Tiger Milk Mushroom Lignosus rhinocerotis (Agaricomycetes) attenuates LPS-induced inflammation in BV2 cells via Nrf2 pathway |
260 | |b Universidade de São Paulo, |c 2020-06-01T00:00:00Z. | ||
500 | |a 2175-9790 | ||
500 | |a 10.1590/s2175-97902020000118499 | ||
520 | |a Lignosus rhinocerotis (tiger milk mushroom) is widely used by the indigenous people of Malaysia as a traditional remedy. The present study was carried out in order to evaluate the antioxidant, cytotoxic and anti-neuroinflammatory activities of L. rhinocerotis extract on brain microglial cells (BV2). The antioxidant activity was evaluated by 2,2-diphenyl-1-picryhydrazyl (DPPH•), 2,2'-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS•+) scavenging assays, and ferric reducing antioxidant power (FRAP). The FRAP, DPPH and ABTS•+ scavenging capacities of the TE3 fraction were 420.77 mg FE/g, 58.01%, and 7%, respectively. The cytotoxic activity was determined by MTS assay. The in vitro model of anti-neuroinflammatory property was evaluated by measuring the production of nitric oxide (NO) in lipopolysaccharide (LPS)-induced BV2 cells. The TE3 fraction showed a significant NO reduction at 1 to 100 µg/mL. The TE3 fraction down-regulated inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX2) genes while it upregulated heme oxygenase (HO-1) and NADPH quinone acceptor oxidoreductase-1 (NQO-1) genes. The nuclear factor (erythroid-derived 2)-like 2 (Nrf2) transcription was also activated. The chemical component of the active fraction (TE3) was identified by gas chromatography-mass spectrometry (GCMS). Overall, the BV2 in vitro model anti-neuroinflammatory activity of L. rhinocerotis may be caused by the lipid constituents identified in the fraction. | ||
546 | |a EN | ||
690 | |a Neuroinflammation | ||
690 | |a Lignosus rhinocerotis | ||
690 | |a Mushroom | ||
690 | |a BV2 cells | ||
690 | |a Nrf2 pathway | ||
690 | |a Pharmacy and materia medica | ||
690 | |a RS1-441 | ||
655 | 7 | |a article |2 local | |
786 | 0 | |n Brazilian Journal of Pharmaceutical Sciences, Vol 56 (2020) | |
787 | 0 | |n http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100646&tlng=en | |
787 | 0 | |n https://doaj.org/toc/2175-9790 | |
856 | 4 | 1 | |u https://doaj.org/article/8a72f7064e4b454886d01ac865da9cf2 |z Connect to this object online. |