Lipid-rich fraction of the sclerotium of Tiger Milk Mushroom Lignosus rhinocerotis (Agaricomycetes) attenuates LPS-induced inflammation in BV2 cells via Nrf2 pathway

Lignosus rhinocerotis (tiger milk mushroom) is widely used by the indigenous people of Malaysia as a traditional remedy. The present study was carried out in order to evaluate the antioxidant, cytotoxic and anti-neuroinflammatory activities of L. rhinocerotis extract on brain microglial cells (BV2)....

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Main Authors: Neeranjini Nallathamby (Author), Hariprasath Lakshmanan (Author), Sharmili Vidyadaran (Author), Sri Nurestri Abdul Malek (Author), Chia-Wei Phan (Author), Vikineswary Sabaratnam (Author)
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Published: Universidade de São Paulo, 2020-06-01T00:00:00Z.
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042 |a dc 
100 1 0 |a Neeranjini Nallathamby  |e author 
700 1 0 |a Hariprasath Lakshmanan  |e author 
700 1 0 |a Sharmili Vidyadaran  |e author 
700 1 0 |a Sri Nurestri Abdul Malek  |e author 
700 1 0 |a Chia-Wei Phan  |e author 
700 1 0 |a Vikineswary Sabaratnam  |e author 
245 0 0 |a Lipid-rich fraction of the sclerotium of Tiger Milk Mushroom Lignosus rhinocerotis (Agaricomycetes) attenuates LPS-induced inflammation in BV2 cells via Nrf2 pathway 
260 |b Universidade de São Paulo,   |c 2020-06-01T00:00:00Z. 
500 |a 2175-9790 
500 |a 10.1590/s2175-97902020000118499 
520 |a Lignosus rhinocerotis (tiger milk mushroom) is widely used by the indigenous people of Malaysia as a traditional remedy. The present study was carried out in order to evaluate the antioxidant, cytotoxic and anti-neuroinflammatory activities of L. rhinocerotis extract on brain microglial cells (BV2). The antioxidant activity was evaluated by 2,2-diphenyl-1-picryhydrazyl (DPPH•), 2,2'-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS•+) scavenging assays, and ferric reducing antioxidant power (FRAP). The FRAP, DPPH and ABTS•+ scavenging capacities of the TE3 fraction were 420.77 mg FE/g, 58.01%, and 7%, respectively. The cytotoxic activity was determined by MTS assay. The in vitro model of anti-neuroinflammatory property was evaluated by measuring the production of nitric oxide (NO) in lipopolysaccharide (LPS)-induced BV2 cells. The TE3 fraction showed a significant NO reduction at 1 to 100 µg/mL. The TE3 fraction down-regulated inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX2) genes while it upregulated heme oxygenase (HO-1) and NADPH quinone acceptor oxidoreductase-1 (NQO-1) genes. The nuclear factor (erythroid-derived 2)-like 2 (Nrf2) transcription was also activated. The chemical component of the active fraction (TE3) was identified by gas chromatography-mass spectrometry (GCMS). Overall, the BV2 in vitro model anti-neuroinflammatory activity of L. rhinocerotis may be caused by the lipid constituents identified in the fraction. 
546 |a EN 
690 |a Neuroinflammation 
690 |a Lignosus rhinocerotis 
690 |a Mushroom 
690 |a BV2 cells 
690 |a Nrf2 pathway 
690 |a Pharmacy and materia medica 
690 |a RS1-441 
655 7 |a article  |2 local 
786 0 |n Brazilian Journal of Pharmaceutical Sciences, Vol 56 (2020) 
787 0 |n http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100646&tlng=en 
787 0 |n https://doaj.org/toc/2175-9790 
856 4 1 |u https://doaj.org/article/8a72f7064e4b454886d01ac865da9cf2  |z Connect to this object online.