Multi-Omics Revealed Resveratrol and β-Hydroxy-β-methyl Butyric Acid Alone or in Combination Improved the Jejunal Function in Tibetan Sheep

Previous research studies confirmed that both resveratrol (RES) and β-hydroxy-β-methyl butyric acid (HMB) improved growth performance by altering intestinal microbiota. However, the mechanism underlying of RES and HMB on intestinal function remains unclear in ruminant. In this study, supplements of...

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Main Authors: Qiurong Ji (Author), Fengshuo Zhang (Author), Yu Zhang (Author), Quyangangmao Su (Author), Tingli He (Author), Shengzhen Hou (Author), Linsheng Gui (Author)
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Published: MDPI AG, 2024-07-01T00:00:00Z.
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042 |a dc 
100 1 0 |a Qiurong Ji  |e author 
700 1 0 |a Fengshuo Zhang  |e author 
700 1 0 |a Yu Zhang  |e author 
700 1 0 |a Quyangangmao Su  |e author 
700 1 0 |a Tingli He  |e author 
700 1 0 |a Shengzhen Hou  |e author 
700 1 0 |a Linsheng Gui  |e author 
245 0 0 |a Multi-Omics Revealed Resveratrol and β-Hydroxy-β-methyl Butyric Acid Alone or in Combination Improved the Jejunal Function in Tibetan Sheep 
260 |b MDPI AG,   |c 2024-07-01T00:00:00Z. 
500 |a 10.3390/antiox13080892 
500 |a 2076-3921 
520 |a Previous research studies confirmed that both resveratrol (RES) and β-hydroxy-β-methyl butyric acid (HMB) improved growth performance by altering intestinal microbiota. However, the mechanism underlying of RES and HMB on intestinal function remains unclear in ruminant. In this study, supplements of RES and HMB alone or in combination were evaluated as promoters of antioxidant capacity, immune response and barrier function, and modulators of the microbiota and metabolite profiles in the jejunum of Tibetan sheep. A total of 120 two-month-old Tibetan rams were randomly divided into four treatments (<i>n</i> = 30 per treatment), which were supplemented with a basal diet with 1.5 g RES/d (RES group), 1.25 g HMB/d (HMB group), 1.5 g RES/d plus 1.25 g HMB/d (RES-HMB group), and without additions (Control group). The results showed that RES and HMB improved the antioxidant capacity (CAT, GSH-Px, SOD, and T-AOC), immunity (IgA, IgG, and IgM), and digestive enzyme activity (α-amylase, lipase, and chymotrypsin) of the experimental lambs (<i>p</i> < 0.05). Additionally, jejunal morphology including villus width, villus height, and muscle layer thickness exhibited a significant difference when rams were fed diets supplemented with RES and HMB (<i>p</i> < 0.05). Furthermore, the determination of fermentation parameters showed that the butyrate concentration in the RES-HMB group was greater than those in the C and RES groups (<i>p</i> < 0.05). When compared to the C group, barrier-related gene expression (<i>MUC-2</i>, <i>ZO-1</i>, and <i>IL-10</i>) was significantly increased in the RES-HMB group (<i>p</i> < 0.05). Dietary RES and (or) HMB supplementation significantly increased the abundance of <i>Methanobrevibacter</i>, <i>Actinobacteriota</i> and <i>Bacillus</i> (<i>p</i> < 0.05). The abundance of differential bacteria was positively associated with butyrate concentration (<i>p</i> < 0.05). Metabolome analysis revealed that alpha ketoglutarate, succinic semialdehyde, and diacetyl as well as butanoate metabolism pathways connected to the improvements in butyrate concentration by RES and (or) HMB supplementation. Collectively, our results suggested that RES and (or) HMB supplementation improved butyrate concentration via regulating the microbial community (<i>Methanobrevibacter</i>, <i>Actinobacteriota</i> and <i>Bacillus</i>) and metabolism (alpha ketoglutarate, succinic semialdehyde, and diacetyl), thus contributing to jejunal morphology, antioxidant capacity, immune response, digestive enzyme activity, and barrier function. 
546 |a EN 
690 |a resveratrol 
690 |a β-hydroxy-β-methyl butyric acid 
690 |a microbiota 
690 |a metabolic profiles 
690 |a Tibetan sheep 
690 |a Therapeutics. Pharmacology 
690 |a RM1-950 
655 7 |a article  |2 local 
786 0 |n Antioxidants, Vol 13, Iss 8, p 892 (2024) 
787 0 |n https://www.mdpi.com/2076-3921/13/8/892 
787 0 |n https://doaj.org/toc/2076-3921 
856 4 1 |u https://doaj.org/article/8af6a33ef51944b2a1e0f74bef4b13b9  |z Connect to this object online.